Applied Biosystems™

BigDye™ Terminator v3.1 Cycle Sequencing Kit

Catalog number:  4337456

Related applications:

SARS-CoV-2 Pathogen Research Solutions

| DNA Sequencing

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Description

The BigDye® Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, plasmid, fosmid, and BAC templates.

• Improve the quality of your results for a wide range of sequencing applications
• Optimized for long read lengths
• Better dye mobility characteristics
• Improved performance reading through GT-rich regions
• Get longer, higher-quality reads with more uniform peak heights and optimal signal balance
• Enhance your productivity and reduce costs

Get more uniform peak heights and improved accuracy
With BigDye® Terminator v3.1 kit superior chemistry, you generate data with uniform peak heights and optimized signal balance. This results in longer, higher-quality reads and more accurate base assignments for heterozygote and mutation detection.
WARNING: Reproductive Harm - www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Application): Ideal for De Novo Sequencing, Resequencing and Finishing with PCR Product, Plasmid, Fosmid and BAC templates
For Use With (Equipment): 310 Genetic Analyzer, 3130xl Genetic Analyzer, 3730xl DNA Analyzer, GeneAmp 9700, Veriti Thermal Cycler, 3130 Genetic Analyzer, 3730 DNA Analyzer, 3500xL Genetic Analyzer, 3500 Genetic Analyzer
Description: Cycle Sequencing Kit, 1000 rxns, Includes 10 Tubes of BigDye™ Terminator v3.1 Ready Reaction Mix, M13 (-21) Primer, pGEM™-3Zf(+) double-stranded DNA Control Template, 1 x 12mL tube of 5X Sequencing Buffer (may be stored at 4°C)
Format: 8-well strips, 12-strip wells, 384-well plate, 96-well plate
Includes: 10 Tubes of BigDye™ Terminator v3.1 Ready Reaction Mix, M13 (-21) Primer, pGEM™-3Zf(+) double-stranded DNA Control Template, 1 x 12mL tube of 5X Sequencing Buffer (may be stored at 4°C)
Recommended Applications: AT-Rich Sequencing (>65%), Comparative Sequencing (Germline Mutations - 50:50), De Novo Sequencing - Low to Mid Throughput, GC-Rich Sequencing (>65%), Standard Sequencing, Long-Read Sequencing, Resequencing, Mixed-Base Detection, GT-Rich or Difficult Template Sequencing
Technique: Fluorescent Dye Terminator Sequencing
Template Compatibility: BAC DNA, Lambda DNA, Genomic DNA (Bacterial), Rolling Circle Amplified Products, Fosmids, PCR Amplicons (50:50 Heterozygotes), Plasmid DNA (≤15Kb), Single Stranded DNA
Product Line: BigDye™

Contents & storage

• 10 tubes of BigDye® Terminator v3.1 Ready Reaction Mix
• M13 (-21) Primer
• pGEM®-3Zf(+) double-stranded DNA Control Template
• 1 × 12 ml tube of 5X Sequencing Buffer (may be stored at 4°C)

Store kit at -15°C to -25°C and avoid repeated freeze-thaw cycles.