Applied Biosystems™

BigDye™ Terminator v3.1 Cycle Sequencing Kit

Catalog number:  4337456

 Related applications:

DNA Sequencing

| Sanger Sequencing

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The BigDye® Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, Plasmid, Fosmid, and BAC templates.
• Improve the quality of your results for a wide range of sequencing applications.
• Optimized for long read lengths.
• Better dye mobility characteristics
• Improved performance reading through GT rich regions.
• Get longer, higher-quality reads with more uniform peak heights and optimal signal balance.
• Enhance your productivity and reduce costs.

Get More Uniform Peak Heights, Improved Accuracy
With BigDye® Terminator v3.1 kit superior chemistry, you generate data with uniform peak heights and optimized signal balance. This results in longer, higher-quality reads and more accurate base assignments for heterozygote and mutation detection.

For Research Use Only. Not for use in diagnostics procedures.
WARNING: Reproductive Harm -
For Research Use Only. Not for use in diagnostic procedures.


For Use With (Equipment): 310 Genetic Analyzer, 3130 Genetic Analyzer, 3130xl Genetic Analyzer, 3500 Genetic Analyzer, 3500xL Genetic Analyzer, 3730 DNA Analyzer, 3730xl DNA Analyzer, GeneAmp 9700, Veriti Thermal Cycler
Recommended Applications: AT-Rich Sequencing (>65%), Comparative Sequencing (Germline Mutations - 50:50), De Novo Sequencing - Low to Mid Throughput, GC-Rich Sequencing (>65%), GT-Rich or Difficult Template Sequencing, Long-Read Sequencing, Mixed-Base Detection, Resequencing, Standard Sequencing
Format: 12-strip wells, 384-well plate, 8-well strips, 96-well plate
Product Line: BigDye™
Product Size: 1,000 reactions
Technique: Fluorescent Dye Terminator Sequencing
Template Compatibility: BAC DNA, Fosmids, Genomic DNA (Bacterial), Lambda DNA, PCR Amplicons (50:50 Heterozygotes), Plasmid DNA (≤15Kb), Rolling Circle Amplified Products, Single Stranded DNA

Contents & storage

• 10 tubes of BigDye® Terminator v3.1 Ready Reaction Mix
• M13 (-21) Primer
• pGEM®-3Zf(+) double-stranded DNA Control Template
• 1 × 12 ml tube of 5X Sequencing Buffer (may be stored at 4°C)

Store kit at -15°C to -25°C and avoid repeated freeze-thaw cycles.


Manuals & protocols