BigDye® Terminator v3.1 Cycle Sequencing Kit

Catalog number: 4337456

Applied Biosystems™  Related applications: DNA Sequencing | Sanger Sequencing

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The BigDye® Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, Plasmid, Fosmid, and BAC templates.
• Improve the quality of your results for a wide range of sequencing applications.
• Optimized for long read lengths.
• Better dye mobility characteristics
• Improved performance reading through GT rich regions.
• Get longer, higher-quality reads with more uniform peak heights and optimal signal balance.
• Enhance your productivity and reduce costs.

Get More Uniform Peak Heights, Improved Accuracy
With BigDye® Terminator v3.1 kit superior chemistry, you generate data with uniform peak heights and optimized signal balance. This results in longer, higher-quality reads and more accurate base assignments for heterozygote and mutation detection.

For Research Use Only. Not for use in diagnostics procedures.
For Research Use Only. Not for use in diagnostic procedures.


For Use With (Equipment): GeneAmp® 9700, 3730 DNA Analyzer, 3500xL Genetic Analyzer, Veriti® Thermal Cycler, 3500 Genetic Analyzer, 3130xl Genetic Analyzer, 310 Genetic Analyzer, 3130 Genetic Analyzer, 3730xl DNA Analyzer
Recommended Applications: AT-Rich Sequencing (>65%), Comparative Sequencing (Germline Mutations - 50:50), De Novo Sequencing - Low to Mid Throughput, GT-Rich or Difficult Template Sequencing, Resequencing, Standard Sequencing, Mixed-Base Detection, Long-Read Sequencing, GC-Rich Sequencing (>65%)
Format: 8-well strips, 384-well plate, 12-strip wells, 96-well plate
Technique: Fluorescent Dye Terminator Sequencing
Product Size: 1,000 reactions
Template Compatibility: BAC DNA, Genomic DNA (Bacterial), Fosmids, Plasmid DNA (≤15Kb), Rolling Circle Amplified Products, PCR Amplicons (50:50 Heterozygotes), Single Stranded DNA, Lambda DNA

Contents & storage

• 10 tubes of BigDye® Terminator v3.1 Ready Reaction Mix
• M13 (-21) Primer
• pGEM®-3Zf(+) double-stranded DNA Control Template
• 1 × 12 ml tube of 5X Sequencing Buffer (may be stored at 4°C)

Store kit at -15°C to -25°C and avoid repeated freeze-thaw cycles.


Manuals & protocols

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