Applied Biosystems™

QuantStudio™ 12K Flex OpenArray™ Human miRNA Starter Kit

Catalog number:  4469606

 Related applications:

Digital PCR

| Real Time PCR (qPCR)

| miRNA & Non-Coding RNA Analysis

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A variety of starter kits are available to introduce you to the various applications of the QuantStudio™ 12K Flex OpenArray® sample block. Each starter kit contains 2 arrays, plus sufficient reagents and accessories to perform two experiments. The miRNA kit also contains cDNA controls to serve as samples for your experiments. Note that the QuantStudio™ 12K Flex Software (included with the QuantStudio™ 12K Flex System) offers example experiment files for each application.

The accessories provided with this kit include: 6 lids and plugs, 6 immersion fluid syringes, 2 carriers, 1 box of Accufill™ System tips, and 384-well plates.


Comments: Use high-quality total RNA samples containing miRNA.
Detection Method: Primer-Probe Detection
Hot Start: Built-In Hot Start
PCR Method: 2-Step qRT-PCR
Polymerase: AmpliTaq Gold DNA Polymerase
Primer-Probe Compatibility: TaqMan®
Product Line: OpenArray®, QuantStudio™
Product Size: 1 kit
Reaction Speed: Standard
Reverse Transcriptase: MultiScribe™
Sample Type (General): RNA
Sample Type (Specific): Total RNA
For Use With (Equipment): QuantStudio™ 12k Flex

Contents & storage

The QuantStudio™ 12K Flex OpenArray® Human miRNA Starter Kit contains:
• TaqMan® MicroRNA Reverse Transcription Kit (200 reactions)
• Megaplex™ RT Primers, Human Pool A v2.1
• Megaplex™ PreAmp Primers, Human Pool A v2.1
• 2X TaqMan® PreAmp Master Mix Kit
• Human brain and lung total RNA, 100 µg (1 mg/mL) each
• 2X TaqMan® OpenArray® Real-Time PCR Master Mix, 1.5 mL
• TaqMan® OpenArray® Human MicroRNA Panels (2 plates)
• QuantStudio™ 12K Flex OpenArray® Accessories Starter Kit
Upon receipt, store frozen unopened kit at -20°C.


Manuals & protocols