AminoLink™ Reductant - FAQs

No, the gel is stored in sodium azide with no loss in activity. Azide is not a primary amine and is rather unreactive.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Both react with primary amines, but AminoLink Coupling Resin is a two-step conjugation. First a Schiff base is formed between the amine and the aldehyde on the resin, which is then reduced to a stable secondary amine with sodium cyanoborohydride, whereas NHS-Activated Agarose utilizes an NHS ester to form an amide bond in a one-step conjugation.

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AminoLink Plus Coupling Resin is activated at a higher level and has higher flow rates than the original AminoLink Coupling Resin, resulting in higher capacity and faster purification. AminoLink Supports can be used to immobilize any molecule with a primary amine.

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AminoLink and AminoLink Plus Supports are activated with aldehyde groups which will react with primary amines to form Schiff bases, which are reduced to stable, non-reversible secondary amines. Coupling efficiency often exceeds 85% with this support.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.