DyLight™ 405 Microscale Antibody Labeling Kit

Catalog number: 53021

Thermo Scientific™  Related applications: Protein Labeling & Crosslinking

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The Thermo Scientific DyLight 405 Microscale Antibody Labeling Kit contains an NHS ester-activated derivative of high-performance DyLight 405 used to fluorescently label antibodies and other proteins that are then used as molecular probes for cellular imaging and other fluorescence detection methods. The microscale kit contains all of the necessary components to perform five separate labeling reactions using 100 µg of IgG.

DyLight 405 has a vibrant blue fluorescence intensity that replaces Alexa Fluor™ 405, Cascade™ Blue and AMCA in many applications. The high water solubility of DyLight Fluors means that a high dye-to-protein ratio can be attained without causing precipitation of the conjugates. DyLight 405 Amine-Reactive Dye is also available as a stand-alone reagent.

Features of the DyLight 405 Amine-Reactive Dye:

High performance— DyLight 405 shows bright fluorescence and replaces Alexa Fluor 405, Cascade Blue and AMCA
Specific— NHS ester-activated dye labels proteins and other molecules at primary amines (-NH2)
Convenient kit sizes— standard and microscale sizes are offered to match your experimental needs
Optimized procedure— following the standard protocol results in antibodies with excellent dye:protein ratios and recovery rates for optimum activity and fluorescence labeling

• Primary antibody labeling for immunofluorescence microscopy, immunohistochemistry (IHC), Western blotting or ELISA assay
• Target protein labeling for in vitro and in vivo fluorescent detection strategies

DyLight 405 Amine-Reactive Dye is activated with an N-hydroxysuccinimide (NHS) ester moiety to react with exposed N-terminal α-amino groups or the ε-amino groups of lysine residues to form stable amide bonds. Learn more about NHS ester chemistry.

Typical labeling reactions require the dye to first be dissolved in anhydrous dimethyl formamide (DMF) or another suitable organic solvent before adding a specific molar amount of dye to an amine-free buffer containing the protein to be labeled. However, the high solubility of DyLight Fluors permits protein solutions to be added directly to specific amounts of the labeling reagent. This feature allows DyLight 405 Amine-Reactive Dye to be provided in multiple formats with flexible protocols to achieve efficient degrees of labeling.

Related Products
DyLight™ 405 NHS Ester
DyLight™ 405 Antibody Labeling Kit
For Research Use Only. Not for use in diagnostic procedures.


Label or Dye: DyLight™ 405
Product Size: 5 reactions
Labeling Scale: 100 µg
Labeling Target: Proteins (General), Antibodies (General)
Excitation⁄Emission (nm): 400⁄420

Contents & storage

Sufficient For: Labeling and purifying 5 x 100 µg (1 mg/mL) of IgG or other proteins
• DyLight Fluor NHS Ester, 5 x 15 µg vials
• Borate Buffer (0.67M), 1 mL
• Purification Resin, 5 mL
• Microcentrifuge Spin Columns, 5 columns
• Microcentrifuge Collection Tubes, 10 tubes

Store the DyLight NHS Ester at -20°C. Store all other kit components at 4°C.


Manuals & protocols