The CaptureSelect™ AAV8 Ligand Leakage ELISA detects affinity ligand contamination of antibodies or antibody fragments purified using POROS™ CaptureSelect™ AAV8 Affinity Resin. In this resin the ligand, a 13 kD recombinant protein, is coupled by multipoint attachment through stable amide bonds to give high chemical stability and low leakage. Even when covalently attached, small amounts of the affinity ligand can leach off of the chromatography support and co-elute with the protein. This ELISA enables the detection of affinity ligand contamination of less than 1 ng/mL. The assay is designed to minimize interference and to provide accurate quantitation even in the presence of the protein of interest. The CaptureSelect AAV8 Ligand Leakage ELISA can be used as a tool to aid in optimal purification process development and in routine quality control of in-process streams as well as final product.
For Research Use Only. Not for use in diagnostic procedures.