Thermo Scientific™

Pierce™ Protein Concentrators PES, 50K MWCO, 0.5–100 mL

Catalog number: 88539
Thermo Scientific™

Pierce™ Protein Concentrators PES, 50K MWCO, 0.5–100 mL

Catalog number: 88539
Easily and quickly concentrate 50K molecular weight cutoff (MWCO) protein and biological samples with Thermo Scientific Pierce Protein Concentrators PES (polyethersulfone), which are disposable centrifugation devices that enable fast sample recovery and a short processing time. These concentrator columns can be used with standard microcentrifuge equipment, and samples can be collected without the need for invert spinning. Samples can be concentrated with or without desalting.
 
Catalog Number
88539
Unit Size
24 concentrators
Sample Volume
2 to 6 mL
Quantity
24 Concentrators
Price (USD)
Price: 158.00
Your Price:
Availability
-
Full specifications
Quantity
DescriptionPierce™ Protein Concentrators PES, 50K MWCO, 2-6 mL
Filter TypeMembrane
Format15 mL Conical, Spin Column
Material (Membrane)PES
MWCO50 kDa
Sample Volume2 to 6 mL
Max. RCFSwinging-bucket rotor: 4,000 x g, Fixed-angle rotor: 10,000 x g
Product LinePierce™
Quantity24 Concentrators
Shipping ConditionRoom Temperature
TypeProtein Concentrator
Unit Size24 concentrators
Catalog Number
Unit Size
25 concentrators
Sample Volume
≤0.5 mL
Quantity
25 Concentrators
Price (USD)
Price: 125.00
Your Price:
Availability
-
Full specifications
Quantity
DescriptionPierce™ Protein Concentrators PES, 50K MWCO, 0.5 mL
Filter TypeMembrane
FormatSpin Column (Microcentrifuge)
Material (Membrane)PES
MWCO50 kDa
Sample Volume≤0.5 mL
Max. RCF15,000 x g
Product LinePierce™
Quantity25 Concentrators
Shipping ConditionRoom Temperature
TypeProtein Concentrator
Unit Size25 concentrators
Catalog Number
Unit Size
10 concentrators
Sample Volume
2 to 6 mL
Quantity
10 Concentrators
Price (USD)
Price: 80.00
Your Price:
Availability
-
Full specifications
Quantity
DescriptionPierce™ Protein Concentrators PES, 50K MWCO, 2-6 mL
Filter TypeMembrane
Format15 mL Conical, Spin Column
Material (Membrane)PES
MWCO50 kDa
Sample Volume2 to 6 mL
Max. RCFSwinging-bucket rotor: 4,000 x g, Fixed-angle rotor: 10,000 x g
Product LinePierce™
Quantity10 Concentrators
Shipping ConditionRoom Temperature
TypeProtein Concentrator
Unit Size10 concentrators
Catalog Number
Unit Size
10 concentrators
Sample Volume
5 to 20 mL
Quantity
10 Concentrators
Price (USD)
Price: 157.00
Your Price:
Availability
-
Full specifications
Quantity
DescriptionPierce™ Protein Concentrators PES, 50K MWCO, 5-20 mL
Filter TypeMembrane
Format50 mL Conical, Spin Column
Material (Membrane)PES
MWCO50 kDa
Sample Volume5 to 20 mL
Max. RCFSwinging-bucket rotor: 5,000 x g, Fixed-angle rotor: 8,000 x g
Product LinePierce™
Quantity10 Concentrators
Shipping ConditionRoom Temperature
TypeProtein Concentrator
Unit Size10 concentrators
Catalog Number
Unit Size
24 concentrators
Sample Volume
5 to 20 mL
Quantity
24 Concentrators
Price (USD)
Price: 317.00
Your Price:
Availability
-
Full specifications
Quantity
DescriptionPierce™ Protein Concentrators PES, 50K MWCO, 5-20 mL
Filter TypeMembrane
Format50 mL Conical, Spin Column
Material (Membrane)PES
MWCO50 kDa
Sample Volume5 to 20 mL
Max. RCFSwinging-bucket rotor: 5,000 x g, Fixed-angle rotor: 8,000 x g
Product LinePierce™
Quantity24 Concentrators
Shipping ConditionRoom Temperature
TypeProtein Concentrator
Unit Size24 concentrators
Catalog Number
Unit Size
4 concentrators
Sample Volume
20 to 100 mL
Quantity
4 Concentrators
Price (USD)
Price: 184.00
Your Price:
Availability
-
Full specifications
Quantity
DescriptionPierce™ Protein Concentrators PES, 50K MWCO, 20-100 mL
Filter TypeMembrane
FormatSpin Column
Material (Membrane)PES
MWCO50 kDa
Sample Volume20 to 100 mL
Max. RCFSwinging-bucket rotor: 2,000 x g, Fixed-angle rotor: not recommended
Product LinePierce™
Quantity4 Concentrators
Shipping ConditionRoom Temperature
TypeProtein Concentrator
Unit Size4 concentrators
Showing 6 of 6
Catalog NumberSpecificationsUnit SizeSample VolumeQuantityPrice (USD)AvailabilityQuantity
88539Full specifications
24 concentrators2 to 6 mL24 Concentrators
Price: 158.00
Your Price:
-
DescriptionPierce™ Protein Concentrators PES, 50K MWCO, 2-6 mL
Filter TypeMembrane
Format15 mL Conical, Spin Column
Material (Membrane)PES
MWCO50 kDa
Sample Volume2 to 6 mL
Max. RCFSwinging-bucket rotor: 4,000 x g, Fixed-angle rotor: 10,000 x g
Product LinePierce™
Quantity24 Concentrators
Shipping ConditionRoom Temperature
TypeProtein Concentrator
Unit Size24 concentrators
88504Full specifications
25 concentrators≤0.5 mL25 Concentrators
Price: 125.00
Your Price:
-
DescriptionPierce™ Protein Concentrators PES, 50K MWCO, 0.5 mL
Filter TypeMembrane
FormatSpin Column (Microcentrifuge)
Material (Membrane)PES
MWCO50 kDa
Sample Volume≤0.5 mL
Max. RCF15,000 x g
Product LinePierce™
Quantity25 Concentrators
Shipping ConditionRoom Temperature
TypeProtein Concentrator
Unit Size25 concentrators
88538Full specifications
10 concentrators2 to 6 mL10 Concentrators
Price: 80.00
Your Price:
-
DescriptionPierce™ Protein Concentrators PES, 50K MWCO, 2-6 mL
Filter TypeMembrane
Format15 mL Conical, Spin Column
Material (Membrane)PES
MWCO50 kDa
Sample Volume2 to 6 mL
Max. RCFSwinging-bucket rotor: 4,000 x g, Fixed-angle rotor: 10,000 x g
Product LinePierce™
Quantity10 Concentrators
Shipping ConditionRoom Temperature
TypeProtein Concentrator
Unit Size10 concentrators
88540Full specifications
10 concentrators5 to 20 mL10 Concentrators
Price: 157.00
Your Price:
-
DescriptionPierce™ Protein Concentrators PES, 50K MWCO, 5-20 mL
Filter TypeMembrane
Format50 mL Conical, Spin Column
Material (Membrane)PES
MWCO50 kDa
Sample Volume5 to 20 mL
Max. RCFSwinging-bucket rotor: 5,000 x g, Fixed-angle rotor: 8,000 x g
Product LinePierce™
Quantity10 Concentrators
Shipping ConditionRoom Temperature
TypeProtein Concentrator
Unit Size10 concentrators
88541Full specifications
24 concentrators5 to 20 mL24 Concentrators
Price: 317.00
Your Price:
-
DescriptionPierce™ Protein Concentrators PES, 50K MWCO, 5-20 mL
Filter TypeMembrane
Format50 mL Conical, Spin Column
Material (Membrane)PES
MWCO50 kDa
Sample Volume5 to 20 mL
Max. RCFSwinging-bucket rotor: 5,000 x g, Fixed-angle rotor: 8,000 x g
Product LinePierce™
Quantity24 Concentrators
Shipping ConditionRoom Temperature
TypeProtein Concentrator
Unit Size24 concentrators
88542Full specifications
4 concentrators20 to 100 mL4 Concentrators
Price: 184.00
Your Price:
-
DescriptionPierce™ Protein Concentrators PES, 50K MWCO, 20-100 mL
Filter TypeMembrane
FormatSpin Column
Material (Membrane)PES
MWCO50 kDa
Sample Volume20 to 100 mL
Max. RCFSwinging-bucket rotor: 2,000 x g, Fixed-angle rotor: not recommended
Product LinePierce™
Quantity4 Concentrators
Shipping ConditionRoom Temperature
TypeProtein Concentrator
Unit Size4 concentrators
Showing 6 of 6
For protein and other biological samples, such as nucleic acids with a 50K MWCO, Thermo Scientific Pierce Protein Concentrators PES offer rapid protein concentration, excellent sample recovery, and instrument compatibility. These centrifugal devices feature a high-flux polyethersulfone (PES) membrane for concentration, desalting, and buffer exchange of biological samples. This product has a molecular-weight cutoff of 50K and can be used for processing sample volumes of 0.5–100 mL.

Features of 50K MWCO Protein Concentrators PES include:
Rapid protein concentration—sample concentration of 10- to 30-fold can be achieved in 15 or fewer minutes
Excellent protein recovery—typical protein recovery is >90% (when the sample mass is at least two-fold greater than the posted MWCO)
Instrument-compatibility—a standard microcentrifuge with swinging (except 88504) or fixed-angle rotors can be used; sample can be collected and recovered without invert spinning
Versatile—easily perform sample concentration, diafiltration, or buffer-exchange of biological samples

For easy identification, the MWCO value is etched on the side of each device. To assist with sample addition and recovery, a clear window with graduations is located on the side of each device enabling visual determination of the retentate volume. The vertical design of the membrane allows high-flux rates, low non-specific binding, and negligible protein aggregation on the membrane and provides reliable and consistent results for processing sample volumes between 0.5 and 100 mL. Typical protein recovery is 90% when the mass of the proteins in the sample is at least two-fold greater than the MWCO of the device used. For example, when using IgG (MW ~150K) samples, use the 5K, 10K, 30K, or 50K MWCO Concentrators. Sample concentration of 10- to 30-fold can be achieved within 15 minutes.

Protein Concentrators PES applications
• Protein concentration of tissue culture media, antiserum, or monoclonal antibody preparations
• Concentration of protein peaks following gel-permeation chromatography
• Removal of unincorporated label following protein modification reactions
• Concentration and desalt/buffer-exchange after elution of protein from ion-exchange, hydrophobic interaction (HIC), metal chelate, or affinity chromatography columns
• Separation of different sized molecules

Pierce Protein Concentrators PES contain a membrane rated for retaining molecules with a molecular weight at least two-fold greater than the MWCO rating of the PES membrane within the device. Reduced recovery may occur when using a concentrator with molecules smaller than the recommended mass. Protein recovery will vary depending on the specific protein in the sample and its starting concentration.

The dead-stop volume for the 0.5 mL, 2–6 mL, 5–20 mL, and 20–100 mL concentrators is approximately 15 µL, 30 µL, 50 µL, and 350 µL, respectively.

To achieve >90% recovery of protein with the 0.5 mL concentrators, the minimum protein sample concentration should be 0.05 mg/mL. For all other concentrators, the minimum protein sample concentration should be 0.1 mg/mL.

Optimal centrifuges and speeds
The 0.5 mL concentrators can be used effectively at a maximum centrifugal force of 15,000 × g using a fixed angle centrifuge only.

For the 2-6 mL concentrators, swinging-bucket or fixed-angle centrifuge models can be used. Swinging bucket centrifuges capable of generating 2,000–4,000 × g and a maximum centrifugal force of 4,000 × g are recommended. With fixed-angle centrifuges, models capable of generating 5,000–10,000 × g and a maximum centrifugal force of 8,000 × g are recommended.

For the 5-20 mL volume concentrators, swinging-bucket or fixed-angle centrifuge models can be used. Swinging-bucket centrifuges capable of generating 2,000–5,000 × g and a maximum centrifugal force of 4,000 × g are recommended. With fixed-angle centrifuges, models capable of generating 5,000–8,000 × g and a maximum centrifugal force of 6,000 × g are recommended.

For the 20-100 mL concentrators, only swinging-bucket centrifuges capable of generating 1,000–2,000 × g are recommended. Use a maximum centrifugal force of 2,000 × g. These concentrators are not recommended for use in fixed-angle rotors.

Centrifugal force, temperature, sample volume, concentration, and viscosity will all affect filtration rate. Centrifugation time should therefore be optimized for each application.

For Research Use Only. Not for use in diagnostic procedures.

Contents & Storage

Store at room temperature.