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Puromycin Dihydrochloride

Catalog number:  A1113802

 Related applications:

Insect Cell Culture

| Mammalian Cell Culture

| Transfection

| Transformation

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Puromycin Dihydrochloride is an aminonucleoside antibiotic produced by Streptomyces alboniger. Puromycin works by inhibiting peptidyl transfer on both prokaryotic and eukaryotic ribosomes; resistance is conferred by the expression of the pac gene.

Puromycin is widely used in cell biology as a selection antibiotic in mammalian cell culture systems. The recommended working concentration ranges from 0.2–5.0 µg/mL, although it can be toxic to eukaryotic cells at concentrations as low as 1 µg/mL. Gibco® Puromycin Dihydrochloride is supplied at 10 mg/mL in 20 mM HEPES buffer (pH 6.2–6.8).

Other Choices and More Information
We offer a wide range of antibiotics and antimycotics in both powder and liquid formats.

See the complete list, or find products for:
Contamination control
Eukaryotic and bacterial selection

See recommendations for working concentrations for selection antibiotics.

Learn more about the use of antibiotics and antimycotics in cell culture, and review guidelines for decontaminating cultures.
For Research Use Only. Not for use in diagnostic procedures.


Validated Application: Eukaryotic Selection⁄Stable Cell Line Generation
Agent: Puromycin
Form: Liquid
Reagent Type: Antibiotic (Selective)
Concentration: 10 mg⁄ml
Formula Weight: 544.43 g⁄mole
Product Line: Gibco®
Product Size: 20 mL
Quantity: 1 bottle
Sterility: Sterile
Shipping Condition: Dry Ice

Contents & storage

Storage conditions: -5 to -20°C
Shipping conditions: Frozen
Shelf life: 12 months from date of manufacture


Manuals & protocols