BacMam LRRK2 G2019S Reagent

Catalog number: A13389

Invitrogen™  Related applications: Pharma & Biopharma | Target & Lead Identification & Validation

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Description

BacMam LRRK2 G2019S Reagent is a modified insect cell virus (baculovirus) that enables easy, efficient, and titratable overexpression of full-length G2019S LRRK2 upregulating mutation in your choice of cellular background, including primary neurons and neuroblastoma cell lines.

BacMam LRRK2 G2019S Reagent Lets You:

• Easily overexpress full-length LRRK2 G2019S in most cellular backgrounds
• Understand the effects of therapeutically relevant LRRK2 mutations
• Conduct the LRRK2 cellular assays you need

Examine LRRK2 Mutations with BacMam Reagents
Using the BacMam LRRK2 therapeutic mutant reagents G2019S and D1994A (Cat. no. A13390) along with the wild-type LRRK2 reagent (Cat. no. A13388) enables you to research and determine LRRK2 mutant mechanisms. You can also evaluate the difference in the cellular response to a stimulus among the different mutations.

Expand Your Battery of Cellular Assays for LRRK2
Once full length LRRK2 G2019S has been introduced into your cellular background, the transduced cells allow you the flexibility to conduct many types of LRRK2 experiments, including studies of regulation of physiologically functional pathways (including signaling pathways leading to function), and identification of potential physiological substrates for LRRK2.

Overexpress Full-Length LRRK2 in Most Mammalian Cell Backgrounds
BacMam technology utilizes a modified baculovirus to deliver and express genes in mammalian cells with minimal effort and toxicity. It enables efficient, titratable LRRK2 G2019S protein expression in various mammalian cellular backgrounds, including cell lines that may not be compatible with traditional transfection methods such as primary neurons and neuroblastoma cell lines.

For research use only. Not intended for any human or animal therapeutic or diagnostic use.

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For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cell State: Cells Not Included
Delivery Method: Baculovirus (BacMam System)
Druggable Target: Kinases
Gene Symbol: LRRK2
Human Protein Class: Kinases
Product Size: 15 ml
Shipping Condition: Wet Ice

Contents & storage

Recombinant baculovirus particles suspended in Gibco® Sf-900™ III SFM media (Cat# 12658-) supplemented with 3% heat-inactivated fetal bovine serum (hiFBS) (Cat# 10100- ). Store at 4°C, keep sterile, protect from light, DO NOT freeze, aliquot upon arrival.

Documents

Manuals & protocols