Bromothymol Blue
Thermo Scientific Chemicals

Bromothymol Blue

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50 g
10 g
250 g
Catalog number A17746.18
also known as A17746-18
Price (USD)/ Each
130.65
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154.00 
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Quantity:
50 g
Request bulk or custom format
Price (USD)/ Each
130.65
Online exclusive
154.00 
Save 23.35 (15%)
Add to cart
Bromothymol Blue
Catalog numberA17746.18
Price (USD)/ Each
130.65
Online exclusive
154.00 
Save 23.35 (15%)
-
Add to cart
Chemical Identifiers
IUPAC Name3,3-bis[3-bromo-4-hydroxy-2-methyl-5-(propan-2-yl)phenyl]-3H-2,1λ⁶-benzoxathiole-1,1-dione
Molecular FormulaC27H28Br2O5S
InChI KeyNUHCTOLBWMJMLX-UHFFFAOYSA-N
SMILESCC(C)C1=CC(=C(C)C(Br)=C1O)C1(OS(=O)(=O)C2=CC=CC=C12)C1=CC(C(C)C)=C(O)C(Br)=C1C
Molecular Weight (g/mol)624.38
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SpecificationsSpecification SheetSpecification Sheet
Specific Absorbance (E 1%/1cm)Lambda-max @ pH 5.8: ≥260
Solution Test(1 mg/L in ethanol): Clear yellow solution
Specific Absorbance (E 1%/1cm)Lambda-max @ pH 7.6: ≥470
Appearance (Color)Pink or brown
Color ChangeYellow @ pH 5.8 to blue @ pH 7.6
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Bromothymol Blue is a pH indicator used for measuring pH around 7 in pools and fish tanks. It operates in the pH range 6.0 to 7.6 due to the presence of an electron withdrawing and donating groups like bromine atoms and alkyl substitutents respectively. In the laboratory, it is used as a biological slide stain as well as used in obstetrics for detecting premature rupture of membranes. Further, it is used for the observation of photosynthetic activities and a respiratory indicator. In addition to this, it is used to define cell walls or nuclei under the microscope.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Applications
Bromothymol Blue is a pH indicator used for measuring pH around 7 in pools and fish tanks. It operates in the pH range 6.0 to 7.6 due to the presence of an electron withdrawing and donating groups like bromine atoms and alkyl substitutents respectively. In the laboratory, it is used as a biological slide stain as well as used in obstetrics for detecting premature rupture of membranes. Further, it is used for the observation of photosynthetic activities and a respiratory indicator. In addition to this, it is used to define cell walls or nuclei under the microscope.

Solubility
Soluble in ethanol, ether and alkalis. Slightly soluble in water.

Notes
Incompatible with strong oxidizing agents.
RUO – Research Use Only

General References:

  1. Acid-base indicator: pH 6.0 - 7.6.
  2. Suwantarat, N.; Watkins, T.; Lee, R.; Carroll, K. C.; Zhang, S. X. False-positive reaction of L-canavanine glycine bromothymol blue medium with Candida famata. J. Clin. Microbiol. 2014, 52 (4), 1308-1309.
  3. El-Nahhal, I. M.; Zourab, S. M.; Kodeh, F. S.; Abdelsalam, F. H. Sol-gel encapsulation of bromothymol blue pH indicator in presence of Gemini 12-2-12 surfactant. J. Sol-Gel Sci. Technol. 2014, 71 (1), 16-23.