I have a goat anti-mouse Alexa Fluor anti-IgM secondary antibody. Will it label my IgG primary antibody as well?
Not likely. With any secondary antibody, you need to consider what other isotypes and species they are cross-adsorbed against. If it is cross-adsorbed against other species or specific chains, cross-reactivity should be minimal or none. Our goat anti-IgM secondary antibodies have been cross-adsorbed against IgG and therefore should exhibit minimal binding to IgG.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.