Characterization of a novel interaction between ELMO1 and ERM proteins.
AuthorsGrimsley CM, Lu M, Haney LB, Kinchen JM, Ravichandran KS
JournalJ Biol Chem
PubMed ID16377631
'ERMs are closely related proteins involved in cell migration, cell adhesion, maintenance of cell shape, and formation of microvilli through their ability to cross-link the plasma membrane with the actin cytoskeleton. ELMO proteins are also known to regulate actin cytoskeleton reorganization through activation of the small GTPbinding protein Rac via ... More
Helicobacter pylori evolution during progression from chronic atrophic gastritis to gastric cancer and its impact on gastric stem cells.
AuthorsGiannakis M, Chen SL, Karam SM, Engstrand L, Gordon JI,
JournalProc Natl Acad Sci U S A
PubMed ID18332421
'We have characterized the adaptations of Helicobacter pylori to a rarely captured event in the evolution of its impact on host biology-the transition from chronic atrophic gastritis (ChAG) to gastric adenocarcinoma-and defined the impact of these adaptations on an intriguing but poorly characterized interaction between this bacterium and gastric epithelial ... More
Src regulates distinct pathways for cell volume control through Vav and phospholipase Cgamma.
AuthorsBarfod ET, Moore AL, Melnick RF, Lidofsky SD
JournalJ Biol Chem
PubMed ID15866884
'Cell volume recovery in response to swelling requires reorganization of the cytoskeleton and fluid efflux. We have previously shown that electrolyte and fluid efflux via K+ and Cl- channels is controlled by swelling-induced activation of phospholipase Cgamma (PLCgamma). Recently, integrin engagement has been suggested to trigger responses to swelling through ... More
The role of Frizzled3 and Frizzled6 in neural tube closure and in the planar polarity of inner-ear sensory hair cells.
AuthorsWang Y, Guo N, Nathans J
JournalJ Neurosci
PubMed ID16495441
'In the mouse, Frizzled3 (Fz3) and Frizzled6 (Fz6) have been shown previously to control axonal growth and guidance in the CNS and hair patterning in the skin, respectively. Here, we report that Fz3 and Fz6 redundantly control neural tube closure and the planar orientation of hair bundles on a subset ... More
Cytokine secretion via cholesterol-rich lipid raft-associated SNAREs at the phagocytic cup.
AuthorsKay JG, Murray RZ, Pagan JK, Stow JL
JournalJ Biol Chem
PubMed ID16513632
'Lipopolysaccharide-activated macrophages rapidly synthesize and secrete tumor necrosis factor alpha (TNFalpha) to prime the immune system. Surface delivery of membrane carrying newly synthesized TNFalpha is controlled and limited by the level of soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) proteins syntaxin 4 and SNAP-23. Many functions in immune cells are ... More
Ultrahigh-resolution imaging reveals formation of neuronal SNARE/Munc18 complexes in situ.
AuthorsPertsinidis A, Mukherjee K, Sharma M, Pang ZP, Park SR, Zhang Y, Brunger AT, Südhof TC, Chu S,
Journal
PubMed ID23821748
'Membrane fusion is mediated by complexes formed by SNAP-receptor (SNARE) and Secretory 1 (Sec1)/mammalian uncoordinated-18 (Munc18)-like (SM) proteins, but it is unclear when and how these complexes assemble. Here we describe an improved two-color fluorescence nanoscopy technique that can achieve effective resolutions of up to 7.5-nm full width at half ... More
Subdiffraction-resolution fluorescence imaging with conventional fluorescent probes.
AuthorsHeilemann M, van de Linde S, Schüttpelz M, Kasper R, Seefeldt B, Mukherjee A, Tinnefeld P, Sauer M,
JournalAngew Chem Int Ed Engl
PubMed ID18646237
Herein we show that conventional fluorophores (Alexa Fluor 647, Cy5) can be used as efficient photoswitchable fluorescent probes. These probes can be reversibly cycled between a fluorescent and a dark state by irradiation with light of different wavelengths without the need for an activator fluorophore. Conceptually analogous to stochastic ... More
Motility of myosin V regulated by the dissociation of single calmodulin.
AuthorsNguyen H, Higuchi H
JournalNat Struct Mol Biol
PubMed ID15665867
Myosin V is a calmodulin-binding motor protein. The dissociation of single calmodulin molecules from individual myosin V molecules at 1 microM Ca(2+) correlates with a reduction in sliding velocity in an in vitro motility assay. The dissociation of two calmodulin molecules at 5 microM Ca(2+) correlates with a detachment of ... More
Investigating cellular structures at the nanoscale with organic fluorophores.
Authorsvan de Linde S, Aufmkolk S, Franke C, Holm T, Klein T, Löschberger A, Proppert S, Wolter S, Sauer M,
JournalChem Biol
PubMed ID23352135
Super-resolution fluorescence imaging can provide insights into cellular structure and organization with a spatial resolution approaching virtually electron microscopy. Among all the different super-resolution methods single-molecule-based localization microscopy could play an exceptional role in the future because it can provide quantitative information, for example, the absolute number of biomolecules interacting ... More
Actin, spectrin, and associated proteins form a periodic cytoskeletal structure in axons.
AuthorsXu K, Zhong G, Zhuang X,
JournalScience
PubMed ID23239625
Actin and spectrin play important roles in neurons, but their organization in axons and dendrites remains unclear. We used stochastic optical reconstruction microscopy to study the organization of actin, spectrin, and associated proteins in neurons. Actin formed ringlike structures that wrapped around the circumference of axons and were evenly spaced ... More
Single-molecule imaging of the functional crosstalk between surface NMDA and dopamine D1 receptors.
AuthorsLadepeche L, Dupuis JP, Bouchet D, Doudnikoff E, Yang L, Campagne Y, Bézard E, Hosy E, Groc L,
Journal
PubMed ID24127604
Dopamine is a powerful modulator of glutamatergic neurotransmission and NMDA receptor-dependent synaptic plasticity. Although several intracellular cascades participating in this functional dialogue have been identified over the last few decades, the molecular crosstalk between surface dopamine and glutamate NMDA receptor (NMDAR) signaling still remains poorly understood. Using a combination of ... More
Test samples for optimizing STORM super-resolution microscopy.
AuthorsMetcalf DJ, Edwards R, Kumarswami N, Knight AE,
Journal
PubMed ID24056752
STORM is a recently developed super-resolution microscopy technique with up to 10 times better resolution than standard fluorescence microscopy techniques. However, as the image is acquired in a very different way than normal, by building up an image molecule-by-molecule, there are some significant challenges for users in trying to optimize ... More
A human CXCL13-induced actin polymerization assay measured by fluorescence plate reader.
AuthorsAlley J, Bloom L, Kasaian M, Gao H, Berstein G, Clark JD, Miao W,
JournalAssay Drug Dev Technol
PubMed ID20085454
The chemokine receptor CXCR5 is predominantly expressed on mature B cells and follicular T-helper cells. CXCR5 and its ligand CXCL13 participate in ectopic germinal center formation at the inflammatory sites of multiple immune diseases such as rheumatoid arthritis, multiple sclerosis, and Sjogren's syndrome. Therefore, disrupting CXCL13-induced chemotaxis may be a ... More
AuthorsColvin RA, Means TK, Diefenbach TJ, Moita LF, Friday RP, Sever S, Campanella GS, Abrazinski T, Manice LA, Moita C, Andrews NW, Wu D, Hacohen N, Luster AD,
JournalNat Immunol
PubMed ID20473299
Chemokines and other chemoattractants direct leukocyte migration and are essential for the development and delivery of immune and inflammatory responses. To probe the molecular mechanisms that underlie chemoattractant-guided migration, we did an RNA-mediated interference screen that identified several members of the synaptotagmin family of calcium-sensing vesicle-fusion proteins as mediators of ... More
Differential regulation of macropinocytosis by Abi1/Hssh3bp1 isoforms.
BACKGROUND: Macropinocytosis, which is a constitutive cellular process of fluid and macromolecule uptake, is regulated by actin cytoskeleton rearrangements near the plasma membrane. Activation of Rac1, which is proposed to act upstream of the actin polymerization regulatory Wave 2 complex, has been found to correlate with enhanced macropinocytosis. One of ... More
Coordination between the actin cytoskeleton and membrane deformation by a novel membrane tubulation domain of PCH proteins is involved in endocytosis.
AuthorsTsujita K, Suetsugu S, Sasaki N, Furutani M, Oikawa T, Takenawa T,
JournalJ Cell Biol
PubMed ID16418535
The conserved FER-CIP4 homology (FCH) domain is found in the pombe Cdc15 homology (PCH) protein family members, including formin-binding protein 17 (FBP17). However, the amino acid sequence homology extends beyond the FCH domain. We have termed this region the extended FC (EFC) domain. We found that FBP17 coordinated membrane deformation ... More
A small molecule agonist of an integrin, alphaLbeta2.
AuthorsYang W, Carman CV, Kim M, Salas A, Shimaoka M, Springer TA
JournalJ Biol Chem
PubMed ID17023419
The binding of integrin alpha(L)beta(2) to its ligand intercellular adhesion molecule-1 is required for immune responses and leukocyte trafficking. Small molecule antagonists of alpha(L)beta(2) are under intense investigation as potential anti-inflammatory drugs. We describe for the first time a small molecule integrin agonist. A previously described alpha/beta I allosteric inhibitor, ... More
Lipids isolated from bone induce the migration of human breast cancer cells.
AuthorsSilva J, Dasgupta S, Wang G, Krishnamurthy K, Ritter E, Bieberich E
JournalJ Lipid Res
PubMed ID16439808
Bone is the most common site to which breast cancer cells metastasize. We found that osteoblast-like MG63 cells and human bone tissue contain the bile acid salt sodium deoxycholate (DC). MG63 cells take up and accumulate DC from the medium, suggesting that the bone-derived DC originates from serum. DC released ... More
Cytoskeletal dynamics and cell signaling during planar polarity establishment in the Drosophila embryonic denticle.
AuthorsPrice MH, Roberts DM, McCartney BM, Jezuit E, Peifer M
JournalJ Cell Sci
PubMed ID16418222
Many epithelial cells are polarized along the plane of the epithelium, a property termed planar cell polarity. The Drosophila wing and eye imaginal discs are the premier models of this process. Many proteins required for polarity establishment and its translation into cytoskeletal polarity were identified from studies of those tissues. ... More
Two distinct distributions of F-actin are present in the hyphal apex of the oomycete Achlya bisexualis.
AuthorsYu YP, Jackson SL, Garrill A
JournalPlant Cell Physiol
PubMed ID15047875
We show that two distinct distributions of F-actin are present in the hyphal apex of the oomycete Achlya bisexualis, that have been chemically fixed with a combination of methylglyoxal and formaldehyde and stained with Alexa phalloidin. In approximately one half of the hyphae examined, an F-actin depleted zone within the ... More
Rac and Cdc42 play distinct roles in regulating PI(3,4,5)P3 and polarity during neutrophil chemotaxis.
AuthorsSrinivasan S, Wang F, Glavas S, Ott A, Hofmann F, Aktories K, Kalman D, Bourne HR
JournalJ Cell Biol
PubMed ID12551955
Neutrophils exposed to chemoattractants polarize and accumulate polymerized actin at the leading edge. In neutrophil-like HL-60 cells, this asymmetry depends on a positive feedback loop in which accumulation of a membrane lipid, phosphatidylinositol (PI) 3,4,5-trisphosphate (PI[3,4,5]P3), leads to activation of Rac and/or Cdc42, and vice versa. We now report that ... More
In vivo negative selection screen identifies genes required for Francisella virulence.
AuthorsWeiss DS, Brotcke A, Henry T, Margolis JJ, Chan K, Monack DM
JournalProc Natl Acad Sci U S A
PubMed ID17389372
Francisella tularensis subverts the immune system to rapidly grow within mammalian hosts, often causing tularemia, a fatal disease. This pathogen targets the cytosol of macrophages where it replicates by using the genes encoded in the Francisella pathogenicity island. However, the bacteria are recognized in the cytosol by the host's ASC/caspase-1 ... More
Intracellular localization and trafficking of fluorescein-labeled cisplatin in human ovarian carcinoma cells.
AuthorsSafaei R, Katano K, Larson BJ, Samimi G, Holzer AK, Naerdemann W, Tomioka M, Goodman M, Howell SB
JournalClin Cancer Res
PubMed ID15701866
PURPOSE: We sought to identify the subcellular compartments in which cisplatin [cis-diamminedichloroplatinum (DDP)] accumulates in human ovarian carcinoma cells and define its export pathways.EXPERIMENTAL DESIGN: Deconvoluting digital microscopy was used to identify the subcellular location of fluorescein-labeled DDP (F-DDP) in 2008 ovarian carcinoma cells stained with organelle-specific markers. Drugs that ... More
Confocal microscopic analysis of the interaction between cisplatin and the copper transporter ATP7B in human ovarian carcinoma cells.
AuthorsKatano K, Safaei R, Samimi G, Holzer A, Tomioka M, Goodman M, Howell SB
JournalClin Cancer Res
PubMed ID15240550
Some cisplatin (DDP)-resistant cells overexpress the copper export transporter ATP7B, and cells molecularly engineered to overexpress ATP7B are resistant to DDP. The interaction of Cu with ATP7B normally triggers its relocalization from the perinuclear region to more peripheral vesicles. To investigate the interaction of DDP with ATP7B, we examined the ... More
Ca2+-activated IK1 channels associate with lipid rafts upon cell swelling and mediate volume recovery.
AuthorsBarfod ET, Moore AL, Roe MW, Lidofsky SD
JournalJ Biol Chem
PubMed ID17264085
Restoration of cell volume in the continued presence of osmotic stimuli is essential, particularly in hepatocytes, which swell upon nutrient uptake. Responses to swelling involve the Ca2+-dependent activation of K+ channels, which promote fluid efflux to drive volume recovery; however, the channels involved in hepatocellular volume regulation have not been ... More
Compartmental signal modulation: Endosomal phosphatidylinositol 3-phosphate controls endosome morphology and selective cargo sorting.
AuthorsFili N, Calleja V, Woscholski R, Parker PJ, Larijani B
JournalProc Natl Acad Sci U S A
PubMed ID17030795
It is increasingly recognized that the compartmental organization of signaling processes has a profound influence on cellular behavior. However, our inability to influence these compartmental events in a spatially restricted and acute manner limits our understanding of causation. To determine whether local compartmental loss of a phosphoinositide disrupts the normal ... More
Focal adhesions in (myo)fibroblasts scaffold adenylyl cyclase with phosphorylated caveolin.
AuthorsSwaney JS, Patel HH, Yokoyama U, Head BP, Roth DM, Insel PA
JournalJ Biol Chem
PubMed ID16618703
Fibroblast-myofibroblast transformation, a critical event for enhanced extracellular matrix deposition, involves formation of an actin stress fiber contractile apparatus that radiates from focal adhesions (FA) in the plasma membrane. Activation of adenylyl cyclase (AC, i.e. increases in cAMP) negatively regulates such transformation. Caveolae and their resident protein caveolins scaffold signaling ... More
AuthorsCulley S, Tosheva KL, Matos Pereira P, Henriques R
JournalInt J Biochem Cell Biol
PubMed ID29852248
'Super-resolution microscopy techniques break the diffraction limit of conventional optical microscopy to achieve resolutions approaching tens of nanometres. The major advantage of such techniques is that they provide resolutions close to those obtainable with electron microscopy while maintaining the benefits of light microscopy such as a wide palette of high ... More
Nuclear and cytoplasmic LIMK1 enhances human breast cancer progression.
Authors
JournalMol Cancer
PubMed ID21682918
Ultrastructure of the axonal periodic scaffold reveals a braid-like organization of actin rings.
AuthorsVassilopoulos S, Gibaud S, Jimenez A, Caillol G, Leterrier C
JournalNat Commun
PubMed ID31862971
'Recent super-resolution microscopy studies have unveiled a periodic scaffold of actin rings regularly spaced by spectrins under the plasma membrane of axons. However, ultrastructural details are unknown, limiting a molecular and mechanistic understanding of these enigmatic structures. Here, we combine platinum-replica electron and optical super-resolution microscopy to investigate the cortical ... More
Long-lived force patterns and deformation waves at repulsive epithelial boundaries.
Authors
JournalNat Mater
PubMed ID28892054
Actomyosin-Mediated Tension Orchestrates Uncoupled Respiration in Adipose Tissues.
Authors
JournalCell Metab
PubMed ID29514068
Network crosstalk dynamically changes during neutrophil polarization.
Authors
JournalCell
PubMed ID22632971
Microglia jointly degrade fibrillar alpha-synuclein cargo by distribution through tunneling nanotubes.
Authors
JournalCell
PubMed ID34555357
Genetic and Mechanical Regulation of Intestinal Smooth Muscle Development.
Traction forces at the cytokinetic ring regulate cell division and polyploidy in the migrating zebrafish epicardium.
Authors
JournalNat Mater
PubMed ID31160803
SARS-CoV-2 Reverse Genetics Reveals a Variable Infection Gradient in the Respiratory Tract.
Authors
JournalCell
PubMed ID32526206
Cell-Intrinsic Control of Interneuron Migration Drives Cortical Morphogenesis.
Authors
JournalCell
PubMed ID29474907
Cell-to-Cell Spreading of HIV-1 in Myeloid Target Cells Escapes SAMHD1 Restriction.
AuthorsXie M, Leroy H, Mascarau R, Woottum M, Dupont M, Ciccone C, Schmitt A, Raynaud-Messina B, Vérollet C, Bouchet J, Bracq L, Benichou S
JournalmBio
PubMed ID31744918
Dendritic cells (DCs) and macrophages as well as osteoclasts (OCs) are emerging as target cells of HIV-1 involved in virus transmission, dissemination, and establishment of persistent tissue virus reservoirs. While these myeloid cells are poorly infected by cell-free viruses because of the high expression levels of cellular restriction factors such ... More
Integrin a6ß4E variant is associated with actin and CD9 structures and modifies the biophysical properties of cell-cell and cell-extracellular matrix interactions.
Integrin a6ß4 is an essential, dynamic adhesion receptor for laminin 332 found on epithelial cells, required for formation of strong cell-extracellular matrix (ECM) adhesion and induced migration, and coordinated by regions of the ß4C cytoplasmic domain. ß4E, a unique splice variant of ß4 expressed in normal tissue, contains a cytoplasmic ... More
The nature and intensity of mechanical stimulation drive different dynamics of MRTF-A nuclear redistribution after actin remodeling in myoblasts.
AuthorsMontel L, Sotiropoulos A, Hénon S
JournalPLoS One
PubMed ID30921405
Serum response factor and its cofactor myocardin-related transcription factor (MRTF) are key elements of muscle-mass adaptation to workload. The transcription of target genes is activated when MRTF is present in the nucleus. The localization of MRTF is controlled by its binding to G-actin. Thus, the pathway can be mechanically activated ... More
Extracellular vesicles originating from glioblastoma cells increase metalloproteinase release by astrocytes: the role of CD147 (EMMPRIN) and ionizing radiation.
AuthorsColangelo NW, Azzam EI
JournalCell Commun Signal
PubMed ID32033611
Glioblastoma multiforme is an aggressive primary brain tumor that is characterized by local invasive growth and resistance to therapy. The role of the microenvironment in glioblastoma invasiveness remains unclear. While carcinomas release CD147, a protein that signals for increased matrix metalloproteinase (MMP) release by fibroblasts, glioblastoma does not have a ... More
A simple DMSO-based method for cryopreservation of primary hippocampal and cortical neurons.
AuthorsIshizuka Y, Bramham CR
JournalJ Neurosci Methods
PubMed ID31899209
Primary neuronal cultures are widely used to elucidate fundamental aspects of neuronal anatomy, physiology, cell biology, and neuronal dysfunction in animal models of disease. However, preparation of primary neuronal cultures from rodent embryos is labor-intensive, and it is often difficult to produce high-quality cultures consistently in a single laboratory, and ... More