RapidFinder™ Pork ID Kit

The RapidFinder™ Pork ID Kit enables the simple and reliable detection of pork in food and feed samples. The method works by detection of porcine, or Sus scrofa, DNA by real-time PCR. Unlike ELISA based methods, when used with the GMO Extraction Kit (Cat. No. 4466336) for sample preparation, the RapidFinder™ Pork ID Kit detects both raw and processed pork and provides sensitivity down to 0.01% porcine DNA. The included positive control, which comprises 0.1% porcine DNA, can be used as a reference to set the threshold of detection of the assay.

• Simple-real-time PCR based assay, no electrophoresis needed
• Flexible-porcine DNA detected in any food or feed sample, both raw and processed
• Reliable-Internal Positive Control (IPC) provides process control check for PCR inhibitors
• Fast-from sample to result in the same day

How the RapidFinder™ Pork ID Kit Works
Porcine DNA detection is accomplished by real-time PCR using the supplied primer/probe set specific for mitochondrial DNA sequence specific to Sus scrofa (including Sus scrofa domesticus, commonly referred to as Sus domesticus). The target sequence, detected in the FAM™ dye channel, is not only specific for pork, but also stable. Also included in each reaction mix is an internal positive control detected in the VIC™ dye channel, which is used to verify the absence of PCR inhibitors.

By performing a separate reaction with the included positive control, users are able to verify the system and assay are working correctly. More importantly, it enables easy data interpretation. The positive control is provided as 0.1% porcine DNA in a background of fish DNA, and by comparing sample results to the control results, a threshold of detection is provided.

The assay is compatible with Applied Biosystems™ QuantStudioTM and 7500 Fast Food Safety Real-Time PCR systems.

Sample Preparation
DNA sample preparation for the RapidFinder™ Pork ID Kit workflow is accomplished with the easy-to-use GMO Extraction Kit (Cat. No. 4466336). Using 10 g of sample rather than the smaller sample sizes accommodated by most other DNA isolation procedures minimizes sampling errors. The GMO Extraction Kit is compatible with raw and processed meat, as well as composite food samples.

Quantification
If a positive result is obtained with the RapidFinder™ Pork ID Kit, most labs will go on to quantify the amount of porcine DNA in the sample. This can be accomplished using the RapidFinder™ Pork ID Kit in conjunction with the RapidFinder™ Quant Multi-Meat Set (Cat. No. A24399).

Part of a Suite of Solutions For Meat Species Detection
The RapidFinder™ Pork ID Kit is just one of our many available kits for the sensitive detection of various meat species in food and feed samples. Other RapidFinder™ meat species identification kits available include those for the detection of goat, beef, horse, rabbit, fallow deer, water buffalo, duck, sheep, ruminant, chicken, poultry, and turkey.

The RapidFinder™ Animal Species Identification kits are made available through a partnership with Health in Code, S.L.