Applied Biosystems™

TaqMan™ Advanced miRNA Assay

Catalog number: A25576
Applied Biosystems™

TaqMan™ Advanced miRNA Assay

Catalog number: A25576
Catalog Number
A25576
Unit Size
S (250 reactions), inventoried
Price (USD)
Price: 328.00
Your Price:
Catalog NumberUnit SizePrice (USD)
A25576S (250 reactions), inventoried
Price: 328.00
Your Price:
Product Overview
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Applied Biosystems TaqMan Advanced miRNA Assays enable highly sensitive and specific quantification of mature miRNAs using qPCR. Together with the TaqMan Advanced miRNA cDNA Synthesis Kit, this solution provides a streamlined upfront workflow that is ideal for analysis of multiple miRNA targets from a single sample or low-level RNA samples such as serum and plasma.

Universal RT—one RT step for all TaqMan Advanced miRNA Assays
Sensitivity—detect as few as 60 copies input into cDNA synthesis
Specificity—detect only the mature miRNA and distinguish between highly homologous targets
Small sample input—detect and quantify mature miRNA from as little as 1 pg total RNA or 2 µL plasma
Compatibility with biofluids including human serum, plasma, and tissue

Pre-formulated assay
• 2 unlabeled PCR primers (900 nM each final 1X concentration)
• 1 FAM dye-labeled TaqMan MGB probe (250 nM final 1X concentration)

TaqMan miRNA Assay selection guide

TaqMan MicroRNA Assays
Description: TaqMan MicroRNA Assays employ a novel target-specific stem–loop primer during cDNA synthesis to produce a template for real-time PCR
RT chemistry: miRNA-specific RT
Throughput: Best for 1–10 targets
Coverage: 205 species available, coverage for miRBase v.21

TaqMan Advanced miRNA Assays
Description: TaqMan Advanced miRNA Assays employ a universal RT step for a streamlined workflow, and a universal miR-Amp step to enable highly sensitive detection by real-time PCR
RT chemistry: Universal RT
Throughput: Best for > 10 targets
Coverage: All human, mouse, and rat miRNAs; coverage for miRBase v.21

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method
Primer-probe
Format
Tube
GC-Rich PCR Performance
High
PCR Method
qPCR
Reaction Speed
Standard
For Use With (Equipment)
7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex
Product Line
TaqMan™
Product Type
Advanced miRNA Assay
Shipping Condition
Room Temperature
Concentration
20X
No. of Reactions
250 Reactions
Volume
2 μL

Contents & Storage

1 tube containing a 20X mix of pre-formulated assay (1 probe and 2 primers).

Store at -15 to -25°C.

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