TaqMan™ Advanced miRNA Assay

Applied Biosystems TaqMan Advanced miRNA Assays enable highly sensitive and specific quantification of mature miRNAs using qPCR.

製品番号（カタログ番号）	数量
A25576	S (250 reactions), inventoried

製品番号（カタログ番号） A25576

価格（JPY）

43,400

S (250 reactions), inventoried

Applied Biosystems TaqMan Advanced miRNA Assays enable highly sensitive and specific quantification of mature miRNAs using qPCR. Together with the TaqMan Advanced miRNA cDNA Synthesis Kit, this solution provides a streamlined upfront workflow that is ideal for analysis of multiple miRNA targets from a single sample or low-level RNA samples such as serum and plasma.

Universal RT—one RT step for all TaqMan Advanced miRNA Assays
Sensitivity—detect as few as 60 copies input into cDNA synthesis
Specificity—detect only the mature miRNA and distinguish between highly homologous targets
Small sample input—detect and quantify mature miRNA from as little as 1 pg total RNA or 2 μL plasma
Compatibility with biofluids including human serum, plasma, and tissue

Pre-formulated assay

2 unlabeled PCR primers (900 nM each final 1X concentration)
1 FAM dye-labeled TaqMan MGB probe (250 nM final 1X concentration)

TaqMan miRNA Assay selection guide

TaqMan MicroRNA Assays

Description: TaqMan MicroRNA Assays employ a novel target-specific stem–loop primer during cDNA synthesis to produce a template for real-time PCR
RT chemistry: miRNA-specific RT
Throughput: Best for 1–10 targets
Coverage: 205 species available, coverage for miRBase v.21

TaqMan Advanced miRNA Assays

Description: TaqMan Advanced miRNA Assays employ a universal RT step for a streamlined workflow, and a universal miR-Amp step to enable highly sensitive detection by real-time PCR
RT chemistry: Universal RT
Throughput: Best for > 10 targets
Coverage: All human, mouse, and rat miRNAs; coverage for miRBase v.21

研究用途にのみご使用ください。診断目的には使用できません。

使用対象 (装置)7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex, QuantStudio™ Absolute Q Digital PCR System

フォーマットTube

反応数250 Reactions

製品ラインTaqMan

製品タイプAdvanced miRNA Assay

数量S (250 reactions), inventoried

出荷条件Room Temperature

容量250 μL

濃度20X

検出法Primer-probe

GC-Rich PCR Performance高

PCR法qPCR

反応速度スタンダード

Unit SizeEach

組成および保存条件

1 tube containing a 20X mix of pre-formulated assay (1 probe and 2 primers).

Store at -15 to -25°C.

よくあるご質問（FAQ）

What is the "alias" associated with the TaqMan miRNA assays?

An alias is the miRBAse ID for a given miRNA from an earlier version. Alias information is found in the Details section of the search results. The miRBase release version shown in parentheses represents when the alias miRBase ID was last listed.

Why is an assay for human named with the name of another species?

An assay for a human miRNA may have a lower species name because the target sequence was first introduced and published in miRBase for that species and only later found in human. For example, mmu-miR-124a was first introduced as a mouse miRNA. The human miRNA was introduced later.

What is the miRBAse accession number?

Accession numbers are unique identifiers assigned to both hairpin (e.g.: MI0000015) and mature (e.g.: MIMAT0000029) sequences. While miRBase names may change, the accession numbers are stable and remain associated with a specific sequence.

What is the miRBase ID?

The mirBase ID is the official miRBase name given to a mature miRNA sequence. There may be multiple miRBase IDs for a given mature miRNA sequence because many miRNAs are conserved across species (for example, several hundred human miRNAs have the identical sequence in common with the mouse species). The miRBase ID consist of a 3 letter species identifier, including the first letter of the genus and the first two letters of the species, followed by the expression “miR”, and then a numeric suffix, e.g.: hsa-miR-212. Since two distinct mature miRNAs can be derived from the different arms of the same stem-loop precursor, a -5p or -3p suffix specifies the particular arm of the stem from which they come, e.g.: hsa-miR-501-5p is derived from the 5' end of the stem, while hsa-miR-501-3p is derived from the 3' end. The miRNA gene and hairpin precursor locus name is given lower case: hsa-mir-212. If a mature miRNA is predicted to be expressed from more than one hairpin precursor, then a numeric suffix is added to the precursor name. For example, hsa-miR-101 is expressed from two precursor loci, hsa-mir-101-1 and hsa-mir-101-2. Closely related miRNAs are given lettered suffixes (hsa-miR-19a-3p and hsa-miR-19b-3p) and are expressed from similarly named precursors (hsa-mir-19a-3p and hsa-mir-19b-3p).

miRBase continues to re-annotate the miRNA sequences as more information becomes available. Updated versions of miRBase are released on a regular basis. At the time of print (June 2013), it was up to v20.

Is the TaqMan Advanced miRNA Assay (Cat. No. A25576) shipped at room temperature?

Yes. Like our other TaqMan assays, the miRNA assays are shipped at ambient temperatures and are stable for multiple days.

Find additional tips, troubleshooting help, and resources within our TaqMan Protein Assay Support Center.

View all TaqMan™ Advanced miRNA Assay FAQs