Applied Biosystems TaqMan Advanced miRNA Assays enable highly sensitive and specific quantification of mature miRNAs using qPCR. Together with the TaqMan Advanced miRNA cDNA Synthesis Kit, this solution provides a streamlined upfront workflow that is ideal for analysis of multiple miRNA targets from a single sample or low-level RNA samples such as serum and plasma.
•
Universal RT—one RT step for all TaqMan Advanced miRNA Assays
•
Sensitivity—detect as few as 60 copies input into cDNA synthesis
•
Specificity—detect only the mature miRNA and distinguish between highly homologous targets
•
Small sample input—detect and quantify mature miRNA from as little as 1 pg total RNA or 2 µL plasma
•
Compatibility with biofluids including human serum, plasma, and tissue
TaqMan miRNA Assay selection guide| | TaqMan MicroRNA Assays | TaqMan Advanced miRNA Assays |
| Description | TaqMan MicroRNA Assays employ a novel target-specific stem–loop primer during cDNA synthesis to produce a template for real-time PCR | TaqMan Advanced miRNA Assays employ a universal RT step for a streamlined workflow, and a universal miR-Amp step to enable highly sensitive detection by real-time PCR |
| RT chemistry | miRNA-specific RT | Universal RT |
| Throughput | Best for 1–10 targets | Best for >10 targets |
| Coverage | 205 species available, coverage for miRBase v.21 | All human, mouse, and rat miRNAs; coverage for miRBase v.21 |
For Research Use Only. Not for use in diagnostic procedures.
