PureLink™ Expi Endotoxin-Free Maxi Plasmid Purification Kit
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PureLink™ Expi Endotoxin-Free Maxi Plasmid Purification Kit
Invitrogen™

PureLink™ Expi Endotoxin-Free Maxi Plasmid Purification Kit

The PureLink Expi Endotoxin-Free Maxi Plasmid Purification Kit enables quick and simple isolation of endotoxin-free plasmid DNA using an enhancedRead more
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Catalog NumberQuantity
A3123125 Preps
A330734 Preps
A3121710 Preps
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Catalog number A31231
Price (USD)/ 25 preps
945.65
Online exclusive
1012.00 
Save 66.35 (7%)
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Add to cart
Quantity:
25 Preps
Price (USD)/ 25 preps
945.65
Online exclusive
1012.00 
Save 66.35 (7%)
Add to cart
PureLink™ Expi Endotoxin-Free Maxi Plasmid Purification Kit
Catalog numberA31231
Price (USD)/ 25 preps
945.65
Online exclusive
1012.00 
Save 66.35 (7%)
-
Add to cart

The PureLink Expi Endotoxin-Free Maxi Plasmid Purification Kit enables quick and simple isolation of endotoxin-free plasmid DNA using an enhanced anion exchange membrane. In as little as 90 minutes, purify up to 1.5 mg of high-quality plasmid DNA that is suitable for advanced transfection applications such as transfection of sensitive cells lines or genome engineering.

Advantages of the PureLink Expi Endotoxin-Free Maxi Plasmid Purification Kit include:
• Simple and fast protocol—purification typically takes 90 minutes
• High yield—isolate up to 1.5 mg of high quality plasmid DNA
• Endotoxin-free plasmid—less than 0.1 EU/μg, ideal for sensitive applications
• High purity—ultralow levels of protein, RNA, and genomic DNA contamination
• Compatibility—suitable for use with ExpiCHO and Expi293 expression systems
• Endotoxin-free components— elution buffer, water, and plasmid storage tubes that are free of endotoxin

Simple and fast protocol
Unlike other anion-exchange-based endotoxin-free plasmid purification systems, the PureLink Expi Endotoxin-Free Maxi Plasmid Purification Kit utilizes centrifugation- or vacuum-assisted filtration for lysate clarification and subsequent plasmid DNA purification. There is no waiting for gravity-flow columns and the entire purification typically takes 90 minutes (including the final precipitation step).

Endotoxin-free plasmid
Purify endotoxin-free (less than 0.1 EU/μg) plasmid that is ideal for sensitive applications such as transfection of primary cells, genome engineering, and research on gene therapy or plasmid vaccines in vivo.

Compatible with ExpiCHO and Expi293 expression systems
Plasmid purified using the PureLink Expi Endotoxin-Free Maxi Plasmid Purification Kit is fully compatible with the Gibco ExpiCHO and Expi293 transient mammalian expression systems.

Superior quality with endotoxin-free components
Rest assured that the kit is of high quality and has additional endotoxin-free elution buffer, water, and plasmid storage tubes for added peace of mind.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
FormatSpin Column
Isolation TechnologyAnion Exchange Resin
Sample TypeBacteria
ScaleMaxi
Expression SystemMammalian
For Use With (Application)Cloning, PCR
High-throughput CompatibilityManual Protocols
No. of Reactions25 Preps
Prep Scale> 1 mg (Large-Scale) Plasmid DNA, > 200 μg (Large-Scale) Plasmid DNA
Product LinePureLink™
Product TypePlasmid Purification Kit
Quantity25 Preps
Shipping ConditionRoom Temperature
TargetPlasmid DNA
Test Time90 min.
Unit Size25 preps
Contents & Storage
• Resuspension buffer
• Lysis indicator
• RNase A
• Lysis buffer
• Precipitation buffer
• Endotoxin removal buffer
• Wash buffer
• Elution buffer
• TE buffer
• Expi Maxi filtration column
• Expi Maxi DNA binding column
• 50 mL conical tubes

Endotoxin-free components: elution buffer, water, plasmid storage tubes