TRIzol™ Reagent and Phasemaker™ Tubes Complete System, 200 Preps - FAQs

View additional product information for TRIzol™ Reagent and Phasemaker™ Tubes Complete System - FAQs (A33251)

5 product FAQs found

Do Phasemaker Tubes affect the performance of downstream applications like RT PCR ?

The polymer in Phasemaker Tubes is inert and heat-stable, and does not affect standard downstream applications performed after RNA extraction.

Can I use Phasemaker Tubes with samples that have been pre-treated with RNAlater?

Yes you can. As RNAlater incorporates salts into the sample, the salt content can increase the density of the sample mix, so we recommend adding RNAse-free water (50-100 µL) to the sample mix.

How do Phasemaker Tubes work?

Phasemaker Tubes work on the principle of density gradient centrifugation. Phasemaker Tubes contain a thick liquid polymer that is heavier than the aqueous phase of the TRIzol Reagent mix, but lighter than the organic phase, so after centrifugation, the polymer positions itself between these two layers and forms an exclusive physical barrier. It separates and completely isolates the upper aqueous phase from the organic phase underneath, making it very easy to aspirate the upper phase containing the RNA.

Can Phasemaker Tubes be used with organic reagents other than TRIzol Reagent?

Phasemaker Tubes work exclusively with TRIzol Reagent (and TRIzol LS Reagent ). As other similar reagents may have different density, Phasemaker Tubes may not work properly.

Can Phasemaker Tubes be used for isolation of DNA or protein?

No. Phasemaker tubes are only validated for RNA extraction.