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IL-12 p70 Human ELISA Kit

Catalog number: EHIL12

Invitrogen™  Related applications: Protein Assays and Analysis

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Description

Assay IL-12 p70 (active human interleukin-12, p70) levels in human serum, plasma (EDTA, heparin, sodium citrate), urine and culture supernatant samples with our Thermo Scientific Human IL-12 (p70) ELISA Kits.

Features of the Human IL-12 ELISA Kit:

Target: biologically active (p70) natural and recombinant human interleukin-12 (IL-12, p70)
Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Measurement: absorbance at 450 nm minus absorbance at 550 nm
Assay Range: 15.4 to 600 pg/mL
Sensitivity: < 3 pg/mL
Precision: intra-assay CV < 10%; inter-assay CV < 10%
Sample Types: cell culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)
Total Assay Time: 3 hours
Sample Size: 50 µL
Specificity: no significant cross-reaction with human IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-13, IL-15, TNFα, IFNγ, GM-CSF, GROα or GROβ. Recombinant human IL-12 (p40) and mouse IL-12 (p40) do not cross-react or interfere with this assay.

These human IL-12 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of biologically active interleukin-12 (IL-12 p70) in samples at 50 µL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Protein Family: Cytokines & Receptors
Assay Range: See product documentation
Assay Sensitivity: See product documentation
Detection Method: Colorimetric
For Use With (Equipment): Microplate Reader
Former Identifiers: Pierce
Gene Aliases: CLMF, CLMF2, IL-12B, IMD28, IMD29, NKSF, NKSF2, CLMF, IL-12A, NFSK, NKSF1, P35
Gene ID: 3592, 3593
Gene Name: interleukin 12A, interleukin 12b
Gene Symbol: IL12A, IL12B
Immunoassay Kit Format: Coated ELISA Kit
Label or Dye: HRP (Horseradish Peroxidase)
Product Size: 96 tests
Protein Name: Interleukin-12 subunit alpha, Interleukin-12 subunit beta
Target (Kit Named): IL-12 p70
Target Species (Named): Human
Target Species (Validated): Human
UniProt ID: P29459, P29460

Contents & storage

Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.

Documents

Manuals & protocols