p53 Human ELISA Kit

Catalog number: EHTP53

Invitrogen™  Related applications: Protein Assays and Analysis

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Description

The Human TP53 (p53) ELISA research-use-only kit is an enzyme-linked immunosorbent assay designed for the quantitation and detection of TP53 (p53) in samples (see sample types indicated) using a microplate reader. The assay will recognize both natural and recombinant forms of this target.

This complete, ready-to-use kit has been developed and optimized to specifically detect TP53 (p53) and includes antibody pre-coated plate(s) and other components to perform the assay. Using the supplied pre-coated, pre-calibrated plate helps reduce variability issues associated with using other kits and assays that require you to coat plates with consistent levels of antibody.

Performance characteristics

• Sensitivity: 0.3 ng/mL
• Standard curve range: 0.3 ng/mL–80 ng/mL
• Sample type(s): cell culture supernatants, plasma, serum
• Specificity: This ELISA antibody pair detect human p53. Other species not determined yet.
• Sample volume: 100 µL per sample after dilution
• Total assay time: 5 hours

Rigorous validation

To help ensure sensitive, accurate, and consistent performance, this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, lot-to-lot consistency, recovery, linearity, and parallelism. See product inserts for further information on validation for this kit.

Learn more about our rigorous ELISA validation and quality testing process.

Principle of the method

This TP53 (p53) solid-phase, sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure and detect the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, binding to the target on a different epitope from the capture antibody. A conjugated enzyme has been incorporated into the assay. After incubation periods and wash steps to remove unbound antibody from the plate, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is proportional to the concentration of target present in the original specimen.

Related links

Learn more about ELISA kits.
Learn more about other immunoassays.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Assay Sensitivity: See product documentation
Detection Method: Colorimetric
For Use With (Equipment): Microplate Reader
Former Identifiers: Pierce
Gene Aliases: BCC7, LFS1, P53, TRP53
Gene ID: 7157
Gene Name: tumor protein p53
Gene Symbol: TP53
Immunoassay Kit Format: Coated ELISA Kit
Label or Dye: HRP (Horseradish Peroxidase)
Product Size: 96 tests
Protein Aliases: Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53
Protein Name: Cellular tumor antigen p53
Shipping Condition: Approved for shipment on Wet or Dry Ice
Target (Kit Named): p53
Target Species (Named): Human
Target Species (Validated): Human
UniProt ID: P04637

Contents & storage

Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.

Documents

Manuals & protocols

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