Thermo Scientific™

DpnI (10 U/µL)

Catalog number: ER1701
Thermo Scientific™

DpnI (10 U/µL)

Catalog number: ER1701
Catalog Number
ER1701
Unit Size
500 units
Price (USD)
42.04
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
2,500 units
Price (USD)
165.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
1,000 units
Price (USD)
72.50
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
ER1701500 units
42.04
Your Price:
***
ER17022,500 units
165.00
Your Price:
***
ER17051,000 units
72.50
Your Price:
***
Product Overview
Figures
Videos
Documents
FAQ
Citations & References
Additional Information
Recommendations
5'  G  Am6T  C   3' 
3'  C  T    ↑Am6G   5' 

Thermo Scientific DpnI restriction enzyme recognizes Gm6A^TC sites and cuts best at 37°C in Tango buffer (isoschizomers: MalI). See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Note: Also available as a FastDigest enzyme for rapid DNA digestion.

Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

Features

• Superior quality—stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
• Wide selection of restriction endonuclease specificities

Applications

• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern blotting
• Restriction fragment length polymorphism (RFLP)
• SNP

Note: DpnI requires the presence of N6-methyladenine within the recognition sequence to cleave DNA. DNA purified from a dam+ strain will be a substrate for DpnI. DpnI will only cleave fully-adenomethylated dam sites. Hemi-adenomethylated dam sites DpnI cleaves 60X more slowly. DpnI, Bsp143I, and MboI all recognize the same sequence, but have different methylation sensitivities and cleavage sites. Assayed using pBR322 DNA. For methylation sensitivity, refer to product specifications.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Methylation Sensitivity
Not CpG methylation-sensitive, dam methylation-sensitive, Not dcm methylation-sensitive
Enzyme
Dpn I
Compatible Buffer
10X Buffer Tango
Sensitive to Heat Inactivation
Yes
Optimal Reaction Temperature
37°C
Type IIS RE
No

Figures

Frequently asked questions (FAQs)

Citations & References