FilmTracer™ Calcein Violet Biofilm Stain

Catalog number: F10320

Molecular Probes™  Related applications: Microbiological Analysis

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F10320 FilmTracer™ calcein violet biofilm stain is an esterase substrate (calcein violet AM) that has utility as a biofilm stain. The stain is non-fluorescent until non-specific esterases hydrolyze the acetoxymethyl ester producing a violet-fluorecent calcein. This esterase substrate has been shown to be converted by Staphylococcus epidermidis organisms, suggesting that the bacteria are all actively producing esterase.
For Research Use Only. Not for use in diagnostic procedures.


Form: Solid
Color: Violet
Label or Dye: CellTrace™ Calcein Violet
Product Size: 20 x 25 µg
Emission Class: Visible
Detection Method: Fluorescent
Excitation Class: Visible
For Use With (Equipment): Microplate Reader, Fluorescence Microscope, Confocal Microscope, Flow Cytometer, Fluorescent Imager
Sub-Cellular Localization: Cytoplasm & Cytosol
Flow Cytometer Laser Lines: 405
Excitation⁄Emission (nm): 400⁄450
Shipping Condition: Room Temperature

Contents & storage

Store in freezer (-5°C to -30°C) protected from light


Manuals & protocols