DBC2, a candidate for a tumor suppressor gene involved in breast cancer.
AuthorsHamaguchi Masaaki; Meth Jennifer L; von Klitzing Christine; Wei Wen; Esposito Diane; Rodgers Linda; Walsh Tom; Welcsh Piri; King Mary-Claire; Wigler Michael H;
JournalProc Natl Acad Sci U S A
PubMed ID12370419
A previously uncharacterized gene, DBC2 (deleted in breast cancer), was cloned from a homozygously deleted region at human chromosome 8p21. DBC2 contains a highly conserved RAS domain and two putative protein interacting domains. Our analyses indicate that DBC2 is the best candidate tumor suppressor gene from this region. It lies ... More
TID1, a human homolog of the Drosophila tumor suppressor l(2)tid, encodes two mitochondrial modulators of apoptosis with opposing functions.
AuthorsSyken J, De-Medina T, Munger K
JournalProc Natl Acad Sci U S A
PubMed ID10411904
'Mitochondria have emerged as central regulators of apoptosis. Here, we show that TID1, a human homolog of the Drosophila tumor suppressor lethal (2) tumorous imaginal discs, l(2)tid, encodes two mitochondrial matrix proteins, designated hTid-1(L) and hTid-1(S). These splice variants are both highly conserved members of the DnaJ family of proteins, ... More
Cell-specific ecdysone-inducible expression of FLP recombinase in mammalian cells.
AuthorsSawicki JA, Monks B, Morris RJ
JournalBiotechniques
PubMed ID9821589
The ability of site-specific recombinases, like FLP and Cre, to catalyze alterations in genomic DNA is well established, whereas their application to genetic engineering strategies has been restricted because of the inability to temporally regulate their expression and subsequent recombination events in specific populations of cells. We describe a regulatory ... More
Exogenous expression of p16INK4a is associated with decrease in telomerase activity.
AuthorsSawa H, Kamada H, Ohshima TA, Noguchi A, Itoh N, Saruta K, Hara M, Saito I
JournalJ Neurooncol
PubMed ID10360478
In this study, gene transfection was used to determine whether the exogenous expression of p16INK4a modulated the biological characteristics of glioblastoma cells. The human glioblastoma cell line U87MG was doubly transfected with the plasmids pVgRXR and pIND harboring the wild-type p16 gene. The expression of p16INK4a in the resulting transfectants ... More