Because it is manufactured at our ISO 9001—certified facilities in Eugene, Oregon, we can guarantee that FluoroPure Grade Hoechst 33342 is greater than or equal to 98% pure by HPLC.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Color
Blue
Excitation Wavelength Range
350⁄461
Dye Type
Hoechst 33342
For Use With (Equipment)
Fluorescence Microscope
Detection Method
Fluorescent
Form
Solid
Product Line
FluoroPure™
Shipping Condition
Room Temperature
Product Type
Stain
SubCellular Localization
Nucleus, Nucleic Acids
Contents & Storage
Store at room temperature and protect from light.
Figures
Osteoblast cells in adult zebrafish head cryosection. ELF 97 Endogenous Phosphatase Detection Kit, Texas Red-X wheat germ agglutinin and Hoechst 33342 nucleic acid stain.
Photobleach protection using ProLong® Live Antifade Reagent
Bovine pulmonary artery endothelial cells (BPAEC) stained with 6-carboxy-2',7'-dichlorodihydrofluorescein diacetate, FM 5-95 and Hoechst 33342.
Bovine pulmonary artery endothelial cells incubated simultaneously with CellTrace™ calcein red-orange AM and MitoTracker® Green FM.
J774 mouse macrophage cell.
A mycoplasma-contaminated African green monkey fibroblast cell line stained with Hoechst 33342 nucleic acid stain.
Stained and Fixed Neonatal Human Dermal Fibroblasts
Live J774 macrophage cells.
Live cell imaging with CellLight reagents
Photobleach protection using ProLong® Live Antifade Reagent
Endogenous phosphatase activity of osteoblast cells in a cartilaginous element of an adult zebrafish head localized using the ELF® 97 Endogenous Phosphatase Detection Kit.
Human metaphase chromosomes hybridized to centromere probes labeled with ChromaTide® OBEA-dCTP nucleotides.
Live J774 macrophage cells labeled with BODIPY® FL C5-ganglioside GM1 and Alexa Fluor® 555 cholera toxin subunit B conjugate.
Viable bovine pulmonary artery endothelial cells simultaneously stained with BODIPY® FL histamine and MitoTracker® Red CMXRos.
Nucleus and Golgi apparatus of a bovine pulmonary artery endothelial cell labeled with Hoechst 33342 and the BSA complex of BODIPY® FL C5-ceramide.
Cultured axolotl sensory neurons stained with our anti–human neuronal protein HuC/HuD antibody and detected using the red-orange–fluorescent Alexa Fluor® 546 goat anti–mouse IgG antibody.
Osteosarcoma cells. Alexa Fluor® 488 concanavalin A, Alexa Fluor® 594 wheat germ agglutinin and Hoechst 33342 nucleic acid stain.
Photobleach protection using ProLong® Live Antifade Reagent
Photobleach protection using ProLong® Live Antifade Reagent
Endogenous alkaline phosphatase activity of osteoblast cells in a cartilaginous element of an adult zebrafish head.
Live-cell labeling using CellMask™ Deep Red plasma membrane stain.
Human metaphase chromosomes hybridized to centromere probes labeled with ChromaTide OBEA-dCTP nucleotides.
Detection of apoptosis in SK-N-MC neuroblastoma cells.
Bovine pulmonary artery endothelial cells stained with the reactive oxygen species indicator, 6-carboxy-2´,7´-dichlorodihydrofluorescein diacetate, di(acetoxymethyl ester) and FM® 5-95.
Photobleach protection using ProLong® Live Antifade Reagent
Centromeres of chromosome 1, chromosome 15 and chromosome 17.
Photobleach protection using ProLong® Live Antifade Reagent
Photobleach protection using ProLong® Live Antifade Reagent
Bovine pulmonary artery endothelial cells. MitoFluor™ Far Red 680, LysoTracker® Green DND-26 and Hoechst 33342.
Nucleus and Golgi apparatus of a bovine pulmonary artery endothelial cell (BPAEC).
Live NIH 3T3 cells labeled with probes for mitochondria, Golgi and the nucleus.
BPAE cells stained with Image-iT® LIVE Mitochondrial Transition Pore Assay Kit.
Viable bovine pulmonary artery endothelial cells incubated simultaneously with MitoFluor™ Red 594 and Hoechst 33342.
Bull sperm. MitoTracker® Green FM and Hoechst 33342.
