Diversity in the neural circuitry of cold sensing revealed by genetic axonal labeling of transient receptor potential melastatin 8 neurons.
AuthorsTakashima Y, Daniels RL, Knowlton W, Teng J, Liman ER, McKemy DD,
JournalJ Neurosci
PubMed ID18094254
'Sensory nerves detect an extensive array of somatosensory stimuli, including environmental temperatures. Despite activating only a small cohort of sensory neurons, cold temperatures generate a variety of distinct sensations that range from pleasantly cool to painfully aching, prickling, and burning. Psychophysical and functional data show that cold responses are mediated ... More
Antiangiogenic activity of 2-deoxy-D-glucose.
AuthorsMerchan JR, Kovács K, Railsback JW, Kurtoglu M, Jing Y, Piña Y, Gao N, Murray TG, Lehrman MA, Lampidis TJ,
JournalPLoS One
PubMed ID21060881
'During tumor angiogenesis, endothelial cells (ECs) are engaged in a number of energy consuming biological processes, such as proliferation, migration, and capillary formation. Since glucose uptake and metabolism are increased to meet this energy need, the effects of the glycolytic inhibitor 2-deoxy-D-glucose (2-DG) on in vitro and in vivo angiogenesis ... More
Early activation, motility, and homing of neonatal microglia to injured neurons does not require protein synthesis.
AuthorsKurpius D, Wilson N, Fuller L, Hoffman A, Dailey ME
JournalGlia
PubMed ID16715500
'Neuronal injury in CNS tissues induces a rapid activation and mobilization of resident microglia (MG). It is widely assumed that changes in gene expression drive the morphological transformation of MG and regulate their mobilization during activation. Here, we used acutely excised neonatal rat brain slices to test whether the morphological ... More
A sensory-labeled line for cold: TRPM8-expressing sensory neurons define the cellular basis for cold, cold pain, and cooling-mediated analgesia.
'Many primary sensory neurons are polymodal, responding to multiple stimulus modalities (chemical, thermal, or mechanical), yet each modality is recognized differently. Although polymodality implies that stimulus encoding occurs in higher centers, such as the spinal cord or brain, recent sensory neuron ablation studies find that behavioral responses to different modalities ... More
Exercise training prevents skeletal muscle afferent sensitization in rats with chronic heart failure.
AuthorsWang HJ, Li YL, Zucker IH, Wang W,
JournalAm J Physiol Regul Integr Comp Physiol
PubMed ID22496362
An exaggerated exercise pressor reflex (EPR) contributes to exercise intolerance and excessive sympathoexcitation in the chronic heart failure (CHF) state, which is prevented by exercise training (ExT) at an early stage in the development of CHF. We hypothesized that ExT has a beneficial effect on the exaggerated EPR by improving ... More
Extracellular matrix proteoglycan plays a pivotal role in sensitization by low pH of mechanosensitive currents in nociceptive sensory neurones.
AuthorsKubo A, Katanosaka K, Mizumura K,
JournalJ Physiol
PubMed ID22570376
Ischaemia, inflammation, and exercise lead to tissue acidosis, which induces pain and mechanical hyperalgesia. Corresponding to this, enhanced thin-fibre afferent responses to mechanical stimulation have been recorded in vitro at low pH. However, knowledge about how this sensitization by low pH occurs is lacking. In this study, we found that ... More
Microglia cells protect neurons by direct engulfment of invading neutrophil granulocytes: a new mechanism of CNS immune privilege.
AuthorsNeumann J, Sauerzweig S, Rönicke R, Gunzer F, Dinkel K, Ullrich O, Gunzer M, Reymann KG,
JournalJ Neurosci
PubMed ID18524901
Microglial cells maintain the immunological integrity of the healthy brain and can exert protection from traumatic injury. During ischemic tissue damage such as stroke, peripheral immune cells acutely infiltrate the brain and may exacerbate neurodegeneration. Whether and how microglia can protect from this insult is unknown. Polymorphonuclear neutrophils (PMNs) are ... More
Microglia promote colonization of brain tissue by breast cancer cells in a Wnt-dependent way.
AuthorsPukrop T, Dehghani F, Chuang HN, Lohaus R, Bayanga K, Heermann S, Regen T, Van Rossum D, Klemm F, Schulz M, Siam L, Hoffmann A, Trümper L, Stadelmann C, Bechmann I, Hanisch UK, Binder C,
JournalGlia
PubMed ID20549749
Although there is increasing evidence that blood-derived macrophages support tumor progression, it is still unclear whether specialized resident macrophages, such as brain microglia, also play a prominent role in metastasis formation. Here, we show that microglia enhance invasion and colonization of brain tissue by breast cancer cells, serving both as ... More
Angiogenesis selectively requires the p110alpha isoform of PI3K to control endothelial cell migration.
AuthorsGraupera M, Guillermet-Guibert J, Foukas LC, Phng LK, Cain RJ, Salpekar A, Pearce W, Meek S, Millan J, Cutillas PR, Smith AJ, Ridley AJ, Ruhrberg C, Gerhardt H, Vanhaesebroeck B,
JournalNature
PubMed ID18449193
Phosphoinositide 3-kinases (PI3Ks) signal downstream of multiple cell-surface receptor types. Class IA PI3K isoforms couple to tyrosine kinases and consist of a p110 catalytic subunit (p110alpha, p110beta or p110delta), constitutively bound to one of five distinct p85 regulatory subunits. PI3Ks have been implicated in angiogenesis, but little is known about ... More
Microglial cells in astroglial cultures: a cautionary note.
AuthorsSaura J,
JournalJ Neuroinflammation
PubMed ID17937799
Primary rodent astroglial-enriched cultures are the most popular model to study astroglial biology in vitro. From the original methods described in the 1970's a great number of minor modifications have been incorporated into these protocols by different laboratories. These protocols result in cultures in which the astrocyte is the predominant ... More
Neutralizing antibody to VEGF reduces intravitreous neovascularization and may not interfere with ongoing intraretinal vascularization in a rat model of retinopathy of prematurity.
AuthorsGeisen P, Peterson LJ, Martiniuk D, Uppal A, Saito Y, Hartnett ME,
JournalMol Vis
PubMed ID18334951
PURPOSE: To study the effects of a neutralizing antibody to vascular endothelial growth factor (VEGF), given as an intravitreous injection, on intravitreous neovascularization (IVNV) and ongoing vascular development of avascular retina in a rat model relevant to human retinopathy of prematurity. METHODS: Newborn Sprague-Dawley rats were exposed to oxygen fluctuations ... More
Visualizing cold spots: TRPM8-expressing sensory neurons and their projections.
AuthorsDhaka A, Earley TJ, Watson J, Patapoutian A,
JournalJ Neurosci
PubMed ID18199758
Environmental stimuli such as temperature and pressure are sensed by dorsal root ganglion (DRG) neurons. DRG neurons are heterogeneous, but molecular markers that identify unique functional subpopulations are mainly lacking. ThermoTRPs are members of the transient receptor potential family of ion channels and are gated by shifts in temperature. TRPM8 ... More
Technical Advance: The rat aorta contains resident mononuclear phagocytes with proliferative capacity and proangiogenic properties.
AuthorsZorzi P, Aplin AC, Smith KD, Nicosia RF,
JournalJ Leukoc Biol
PubMed ID20628067
Angiogenesis in the aortic ring model is preceded by activation of the immune system and impaired by ablation of adventitial macrophages. Treatment of aortic cultures with M-CSF induced extensive periaortic outgrowth of CD45(+) CD68(+) mononuclear cells with ultrastructural features of macrophages and DCs. Periaortic lysis of collagen caused many CD45(+) ... More
Distinct signals from the microbiota promote different aspects of zebrafish gut differentiation.
AuthorsBates JM, Mittge E, Kuhlman J, Baden KN, Cheesman SE, Guillemin K
JournalDev Biol
PubMed ID16781702
All animals exist in intimate associations with microorganisms that play important roles in the hosts' normal development and tissue physiology. In vertebrates, the most populous and complex community of microbes resides in the digestive tract. Here, we describe the establishment of the gut microbiota and its role in digestive tract ... More
Epac mediates a cAMP-to-PKC signaling in inflammatory pain: an isolectin B4(+) neuron-specific mechanism.
AuthorsHucho TB, Dina OA, Levine JD
JournalJ Neurosci
PubMed ID15987941
The epsilon isoform of protein kinase C (PKCepsilon) has emerged as a critical second messenger in sensitization toward mechanical stimulation in models of neuropathic (diabetes, alcoholism, and cancer therapy) as well as acute and chronic inflammatory pain. Signaling pathways leading to activation of PKCepsilon remain unknown. Recent results indicate signaling ... More
Migration of perilesional microglia after focal brain injury and modulation by CC chemokine receptor 5: an in situ time-lapse confocal imaging study.
AuthorsCarbonell WS, Murase S, Horwitz AF, Mandell JW
JournalJ Neurosci
PubMed ID16049180
Microglia rapidly become reactive in response to diverse stimuli and are thought to be prominent participants in the pathophysiology of both acute injury and chronic neurological diseases. However, mature microglial reactions to a focal lesion have not been characterized dynamically in adult vertebrate tissue. Here, we present a detailed analysis ... More
Human genome-edited hematopoietic stem cells phenotypically correct Mucopolysaccharidosis type I.
AuthorsGomez-Ospina N, Scharenberg SG, Mostrel N, Bak RO, Mantri S, Quadros RM, Gurumurthy CB, Lee C, Bao G, Suarez CJ, Khan S, Sawamoto K, Tomatsu S, Raj N, Attardi LD, Aurelian L, Porteus MH
JournalNat Commun
PubMed ID31492863
'Lysosomal enzyme deficiencies comprise a large group of genetic disorders that generally lack effective treatments. A potential treatment approach is to engineer the patient''s own hematopoietic system to express high levels of the deficient enzyme, thereby correcting the biochemical defect and halting disease progression. Here, we present an efficient ex ... More
Soft, stretchable, fully implantable miniaturized optoelectronic systems for wireless optogenetics.
Authors
JournalNat Biotechnol
PubMed ID26551059
Isolation and culture of endothelial cells, pericytes and perivascular resident macrophage-like melanocytes from the young mouse ear.
Authors
JournalNat Protoc
PubMed ID23493068
Motor neurons control blood vessel patterning in the developing spinal cord.
Authors
JournalNat Commun
PubMed ID28262664
Oligodendrocyte precursor cell specification is regulated by bidirectional neural progenitor-endothelial cell crosstalk.
Authors
JournalNat Neurosci
PubMed ID33510480
Lack of Diaph3 relaxes the spindle checkpoint causing the loss of neural progenitors.
Authors
JournalNat Commun
PubMed ID27848932
Piezo2 senses airway stretch and mediates lung inflation-induced apnoea.
Authors
JournalNature
PubMed ID28002412
A junctional PACSIN2/EHD4/MICAL-L1 complex coordinates VE-cadherin trafficking for endothelial migration and angiogenesis.
Authors
JournalNat Commun
PubMed ID33972531
Exercise-induced angiogenesis is dependent on metabolically primed ATF3/4+ endothelial cells.
Authors
JournalCell Metab
PubMed ID34358431
Neuropathic pain caused by miswiring and abnormal end organ targeting.
Authors
JournalNature
PubMed ID35614217
Pericyte-to-endothelial cell signaling via vitronectin-integrin regulates blood-CNS barrier.
Authors
JournalNeuron
PubMed ID35294899
Antiviral Drug Ganciclovir Is a Potent Inhibitor of the Proliferation of Müller Glia-Derived Progenitors During Zebrafish Retinal Regeneration.
AuthorsZhang S, Mu Z, He C, Zhou M, Liu D, Zhao XF, Goldman D, Xu H
JournalInvest Ophthalmol Vis Sci
PubMed ID27096757
The purpose of this study was to investigate the effect of the antiviral drug ganciclovir (GCV) on Müller glia dedifferentiation and proliferation and the underlying cellular and molecular mechanisms in adult zebrafish. ... More
Persistent reduction in sialylation of cerebral glycoproteins following postnatal inflammatory exposure.
AuthorsDemina EP, Pierre WC, Nguyen ALA, Londono I, Reiz B, Zou C, Chakraberty R, Cairo CW, Pshezhetsky AV, Lodygensky GA
JournalJ Neuroinflammation
PubMed ID30518374
The extension of sepsis encompassing the preterm newborn's brain is often overlooked due to technical challenges in this highly vulnerable population, yet it leads to substantial long-term neurodevelopmental disabilities. In this study, we demonstrate how neonatal neuroinflammation following postnatal E. coli lipopolysaccharide (LPS) exposure in rat pups results in persistent ... More
Utilizing Confocal Microscopy to Characterize Human and Mouse Adipose Tissue.
AuthorsBlackshear CP, Borrelli MR, Shen EZ, Ransom RC, Chung NN, Vistnes SM, Irizarry D, Nazerali R, Momeni A, Longaker MT, Wan DC
JournalTissue Eng Part C Methods
PubMed ID30215305
Significant advances in our understanding of human obesity, endocrinology, and metabolism have been made possible by murine comparative models, in which anatomically analogous fat depots are utilized; however, current research has questioned how truly analogous these depots are. In this study, we assess the validity of the analogy from the ... More
Glucagon-like Peptide 1 Receptor Agonists, Diabetic Retinopathy and Angiogenesis: The AngioSafe Type 2 Diabetes Study.
AuthorsGaborit B, Julla JB, Besbes S, Proust M, Vincentelli C, Alos B, Ancel P, Alzaid F, Garcia R, Mailly P, Sabatier F, Righini M, Gascon P, Matonti F, Houssays M, Goumidi L, Vignaud L, Guillonneau X, Erginay A, Dupas B, Marie-Louise J, Autié M, Vidal-Trecan T, Riveline JP, Venteclef N, Massin P, Muller L, Dutour A, Gautier JF, Germain S
JournalJ Clin Endocrinol Metab
PubMed ID31589290
Recent trials provide conflicting results on the association between glucagon-like peptide 1 receptor agonists (GLP-1RA) and diabetic retinopathy (DR). The aim of the AngioSafe type 2 diabetes (T2D) study was to determine the role of GLP-1RA in angiogenesis using clinical and preclinical models. ... More