iBlot™ Transfer Stack, nitrocellulose, regular size
Limited time savings on remaining stock of iBlot 2 devices! While supplies last. Save now!
Like our original iBlot Dry Blotting System? Then you'll love our iBlot 3 Dry Blotting System which enables twice the throughput and features optional built-in cooling for better consistency. Learn more about the benefits of the iBlot 3 here.
iBlot™ Transfer Stack, nitrocellulose, regular size
Invitrogen™

iBlot™ Transfer Stack, nitrocellulose, regular size

Green features
The iBlot™ Transfer Stacks are used to transfer proteins using the iBlot™ Gel Transfer Device. The iBlot™ Transfer Stacks areRead more
Have Questions?
Change viewbuttonViewtableView
Catalog NumberDimensions (LxW)Quantity
IB301001Regular, 13.5 cm x 8 cm10 sets
IB301031Regular, 13.5 cm x 8 cm3 x 10 sets
Catalog number IB301001
Price (USD)
353.00
Each
Add to cart
Dimensions (LxW):
Regular, 13.5 cm x 8 cm
Quantity:
10 sets
Price (USD)
353.00
Each
Add to cart
The iBlot™ Transfer Stacks are used to transfer proteins using the iBlot™ Gel Transfer Device. The iBlot™ Transfer Stacks are disposable stacks that come in three varieties for different applications: iBlot™ Gel Transfer Stacks have integrated PVDF or nitrocellulose transfer membranes to perform dry blotting of proteins, iBlot™ DNA Transfer Stacks have an integrated nylon transfer membrane to perform dry blotting of DNA, and iBlot™ Western Detection Stacks are used to perform Western detection on previously blotted nitrocellulose or PVDF membranes. Each iBlot™ Transfer Stack contains a copper electrode and appropriate cathode and anode buffers in the gel matrix to allow fast, reliable transfer of proteins or DNA.

Nitrocellulose membrane
The nitrocellulose membrane (0.2 μm pore size) included in this stack is composed of 100% pure nitrocellulose to provide high-quality transfer. The membrane is compatible with commonly used detection methods such as staining, immunodetection, fluorescence, or radiolabeling. The proteins bind to the membrane due to hydrophobic and electrostatic interactions. The protein binding capacity is 209 μg/cm2.

Transfer stack sizes
The regular size (13 cm x 8.3 cm) is used for all Novex™ midi-gel types (or equivalent) and E-PAGE™ 48 or 96 Gels. The mini size (8 cm x 8 cm) is used for all Novex™ mini-gels (or equivalent).
WARNING: Reproductive Harm - www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Green FeaturesSustainable disposal
Quantity10 sets
Shipping ConditionRoom Temperature
Dimensions (LxW)Regular, 13.5 cm x 8 cm
FormatTransfer Stack
Length (Metric)13.5 cm
MaterialNitrocellulose
Pore Size0.2 μm
Product LineiBlot
Sufficient For10 Blots
Width (Metric)8 cm
Unit SizeEach
Contents & Storage
10 sets of transfer stacks in 1 box. Store at room temperature.

Frequently asked questions (FAQs)

Following the release of the iBlot 2 Gel Transfer Device, are consumables for the original iBlot Gel Transfer Device still available?

Yes, all original iBlot stacks are still available for purchase. You can find them in the original iBlot Gel Transfer Device Reagents and Resources (http://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/transfer-proteins-western-blot/iblot-dry-blotting-system/reagents-resources-original-iblot-gel-transfer-device.html). However, please note that they are not compatible with the iBlot 2 Gel transfer Device.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Is the iBlot 2 Gel Transfer Device compatible with original iBlot Transfer Stacks?

No. Do not use iBlot Transfer Stacks in the iBlot 2 Gel Transfer Device, and do not mix components between iBlot Transfer Stacks and iBlot 2 Transfer Stacks.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

How can I get better transfer of high-molecular weight proteins?

Proteins larger than ~150 kDa migrate more slowly than smaller proteins. Therefore, more time is required to transfer them from gel to membrane. We recommend extending the transfer time by 8 to 10 minutes for optimal transfer of proteins >150 kDa using the iBlot Dry Blotting System.

To enhance transfer efficiency, we also recommend adding an equilibration (gel-soaking) step between electrophoresis and western transfer and using NuPAGE Invitrogen 3 - 8% Tris-acetate gels for electrophoresis. We have an application note available, titled “Transferring Large and Small Proteins Using the iBlot Dry Blotting System”. To download the PDF, see the iBlot Reagents Resources page. (http://www.thermofisher.com/us/en/home/life-science/protein-expression-and-analysis/western-blotting/western-blot-transfer/iblot-dry-blotting-system/original-iblot-resources.html)

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Occasionally my western blots have high background. What do you recommend?

This may be a result of insufficient blocking or nonspecific binding. We suggest trying our WesternBreeze Blocker/Diluent (Cat. No. WB7050). We have been using it with good success. Additionally, you should optimize primary and secondary antibody concentrations as generally recommended for any new blotting technique. Many cases of high background can be resolved by further diluting one or both antibody preparations.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Is it possible to substitute the membrane from the iBlot Transfer Stack with my specialized membrane?

In theory, you can replace the membrane provided in your iBlot Transfer Stack with any membrane that is compatible with western blotting. To do this, cut the alternative membrane to match the size of your gel, and wet the membrane. Then, either place the alternative membrane on top of the integrated membrane, or carefully remove the integrated membrane from the gel matrix with forceps and replace it with the new membrane. Note that we only support the use of iBlot Transfer Stacks when they are used with the provided instructions.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

View all iBlot™ Transfer Stack, nitrocellulose, regular size FAQs