Alkaline Phosphatase, calf intestine, EIA Grade

Name: Alkaline Phosphatase, calf intestine, EIA Grade
SKU: J61037.MCR

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数量:

1mg

5mg

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製品番号（カタログ番号） J61037.MCR

または、製品番号J61037-M^

価格（JPY）

数量:

1mg

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化学物質識別子

CAS9001-78-9

IUPAC Name2-{[1-(2-{[1-(2-aminopropanoyl)pyrrolidin-2-yl]formamido}acetyl)pyrrolidin-2-yl]formamido}-5-[(diaminomethylidene)amino]pentanoic acid

Molecular FormulaC21H36N8O6

InChI KeyITZMJCSORYKOSI-UHFFFAOYNA-N

SMILESCC(N)C(=O)N1CCCC1C(=O)NCC(=O)N1CCCC1C(=O)NC(CCCN=C(N)N)C(O)=O

さらに表示

仕様

スペックシート

RNaseNone detected

units/mgPnot less than 3,000 units/mg protein

DNaseNone detected

Alkaline Phosphatase, calf intestine, EIA GradeDephosphorylation of cloning vector DNA to prevent recircularization during ligation, dephosphorylation of DNA prior to end-labeling using T4 Polynucleotide Kinase, treatment of dNTPs in PCR reactions prior to sequencing or SNP analysis, dephosphorylation of DNA and RNA. It is also used to remove the 5?-terminal phosphate from nucleic acids during molecular cloning reactions.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

RUO – Research Use Only

組成および保存条件

Store at -20°C

よくあるご質問（FAQ）

What are the applications of BioAcryl-P (30%, 37.5:1) (Cat. No. J61505.K2, J61505-K2)?

BioAcryl-P (30%, 37.5:1) is suitable for conjugation to antibodies and other proteins for ELISA, Western blotting, and other histochemical procedures. Alkaline phosphatase may be used to dephosphorylate the 5' ends of DNA or RNA. It can also be used to dephosphorylate proteins.

What is the specific activity of BioAcryl-P (30%, 37.5:1) (Cat. No. J61505.K2, J61505-K2)?

The specific activity of BioAcryl-P (30%, 37.5:1) is ≥3,000 units/mg protein (DEA units).

What is the enzyme affinity (Km) of BioAcryl-P (30%, 37.5:1) (Cat. No. J61505.K2, J61505-K2)?

The Km is 1.5 x 10^-3 M (p-Nitrophenyl phosphate); 19 x 10^-3 M (phosphoenolypyruvate).

What is the extinction coefficient of BioAcryl-P (30%, 37.5:1) (Cat. No. J61505.K2, J61505-K2)?

The extinction coefficient (278nm) of BioAcryl-P (30%, 37.5:1) is 7.6-10.5.

What is the molecular mass of BioAcryl-P (30%, 37.5:1) (Cat. No. J61505.K2, J61505-K2)?

The molecular mass of BioAcryl-P (30%, 37.5:1) is ~ 140 kDa.

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