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Thermo Scientific Chemicals
Neutral Red, ACS
Nuclear counterstain useful in pH range 6.8 to 8.0 | CAS: 553-24-2 | C15H17ClN4 | 288.779 g/mol
化学物質識別子
CAS553-24-2
IUPAC Namehydrogen N2,N2,7-trimethylphenazine-2,8-diamine chloride
Molecular FormulaC15H17ClN4
InChI KeyPGSADBUBUOPOJS-UHFFFAOYSA-N
SMILES[H+].[Cl-].CN(C)C1=CC=C2N=C3C=C(C)C(N)=CC3=NC2=C1
さらに表示
仕様
スペックシート
スペックシートAppearance (Color)Dark green to black
Solution TestClear red soluiton (0.1% in 50% ethanol + 2ml acetic acid)
Visual transition intervalRed @ pH 6.8 to yellow @ pH 8.0
FormPowder
Assay from Supplier's CofADye content: ≥50%
Neutral Red is most commonly used for histological stain for lysosymes but has also been incorporated in investigation of viruses, as a pH indicator, determination of DNA with optical and electrochemical methods, evaluation of synthetic and naturally derived biomaterials. Neutral red can be electrochemically polymerized and used as a redox mediator. The compound once taken up by cells can be used to acertain cell viability with a excitation maximum at 470 nm and emission maximum at 580nm. It is used as a red nuclear counter stain in various cytochemical procedures. It is suitable for microscopy and microbiology and supravital staining of living blood cells. A vital dye used as an indicator and biological stain.
This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.
Applications
Neutral Red is most commonly used for histological stain for lysosymes but has also been incorporated in investigation of viruses, as a pH indicator, determination of DNA with optical and electrochemical methods, evaluation of synthetic and naturally derived biomaterials. Neutral red can be electrochemically polymerized and used as a redox mediator. The compound once taken up by cells can be used to acertain cell viability with a excitation maximum at 470 nm and emission maximum at 580nm. It is used as a red nuclear counter stain in various cytochemical procedures. It is suitable for microscopy and microbiology and supravital staining of living blood cells. A vital dye used as an indicator and biological stain.
Solubility
Soluble in water (50 mg/ml at 25°C), abs. alcohol (1.8%), and cello solve (3.75%).
Notes
Store in cool place. Keep container tightly closed in a dry and well-ventilated place. Recommended storage temperature: 2 - 8°C
Neutral Red is most commonly used for histological stain for lysosymes but has also been incorporated in investigation of viruses, as a pH indicator, determination of DNA with optical and electrochemical methods, evaluation of synthetic and naturally derived biomaterials. Neutral red can be electrochemically polymerized and used as a redox mediator. The compound once taken up by cells can be used to acertain cell viability with a excitation maximum at 470 nm and emission maximum at 580nm. It is used as a red nuclear counter stain in various cytochemical procedures. It is suitable for microscopy and microbiology and supravital staining of living blood cells. A vital dye used as an indicator and biological stain.
Solubility
Soluble in water (50 mg/ml at 25°C), abs. alcohol (1.8%), and cello solve (3.75%).
Notes
Store in cool place. Keep container tightly closed in a dry and well-ventilated place. Recommended storage temperature: 2 - 8°C
RUO – Research Use Only
General References:
- Antal, P., et al. Flow cytometric assay of phagocytic activity of human neutrophils and monocytes in whole blood by neutral red uptake. Ann. Hematol. 1995, 70, (5), 259-65.
- Ricci, V., et al. Extracellular pH modulates Helicobacter pylori-induced vacuolation and VacA toxin internalization in human gastric epithelial cells. Biochem. Biophys. Res. Commun. 2002, 292, (1), 167-74.