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Thermo Scientific Chemicals
Hygromycin B, 50 mg/ml in distilled water, sterile-filtered
CAS: 31282-04-9 | C20H37N3O13 | 527.524 g/mol
化学物質識別子
CAS31282-04-9
IUPAC Name(3'R,3aS,4S,4'R,5'R,6R,6'R,7S,7aS)-4-{[(1R,2S,3R,5S,6R)-3-amino-2,6-dihydroxy-5-(methylamino)cyclohexyl]oxy}-6'-[(1S)-1-amino-2-hydroxyethyl]-6-(hydroxymethyl)-tetrahydro-3aH-spiro[[1,3]dioxolo[4,5-c]pyran-2,2'-oxane]-3',4',5',7-tetrol
Molecular FormulaC20H37N3O13
InChI KeyGRRNUXAQVGOGFE-KPBUCVLVSA-N
SMILESCN[C@H]1C[C@@H](N)[C@H](O)[C@@H](O[C@@H]2O[C@H](CO)[C@H](O)[C@@H]3OC4(O[C@H]23)O[C@H]([C@@H](N)CO)[C@H](O)[C@@H](O)[C@H]4O)[C@@H]1O
さらに表示
仕様
スペックシート
スペックシートCommentSterile-filtered
FormLiquid
Concentration50mg/ml
Appearance (Color)Pale yellow to brown
Hygromycin B was used for hygro resistance effect in the following cells transfected 293T cells with mixture of plasmids at 160 ?g/ml, MCF-7ALOX12 cells at 150 ?g/ml, MSCV Luciferase PGK-hygro plasmid and STAT3 Reporter HeLa Stable Cell Line (stably transfected with Luciferase gene under the control of STAT3 promoter) maintained in DMEM supplemented with 10% FBS. It is suitable for the selection of cells transfected with Escherichia coli hygromycin resistance gene (hyg or hph), as well as for the maintenance of the Hygromycin phenotype of resistant cells. Consistent with its more specific effect on translocation, hygromycin B exhibits monophasic inhibition of polypeptide synthesis, suggesting that it binds to a single site on the ribosome.
This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.
Applications
Hygromycin B was used for hygro resistance effect in the following cells transfected 293T cells with mixture of plasmids at 160 μg/ml, MCF-7ALOX12 cells at 150 μg/ml, MSCV Luciferase PGK-hygro plasmid and STAT3 Reporter HeLa Stable Cell Line (stably transfected with Luciferase gene under the control of STAT3 promoter) maintained in DMEM supplemented with 10% FBS. It is suitable for the selection of cells transfected with Escherichia coli hygromycin resistance gene (hyg or hph), as well as for the maintenance of the Hygromycin phenotype of resistant cells. Consistent with its more specific effect on translocation, hygromycin B exhibits monophasic inhibition of polypeptide synthesis, suggesting that it binds to a single site on the ribosome.
Solubility
Fully miscible in water and also soluble in methanol or ethanol and buffer solution.
Notes
Protect from heat. Keep the container tightly closed and place it in a cool, dry and well ventilated condition. Store in freezer at -20°C.
Hygromycin B was used for hygro resistance effect in the following cells transfected 293T cells with mixture of plasmids at 160 μg/ml, MCF-7ALOX12 cells at 150 μg/ml, MSCV Luciferase PGK-hygro plasmid and STAT3 Reporter HeLa Stable Cell Line (stably transfected with Luciferase gene under the control of STAT3 promoter) maintained in DMEM supplemented with 10% FBS. It is suitable for the selection of cells transfected with Escherichia coli hygromycin resistance gene (hyg or hph), as well as for the maintenance of the Hygromycin phenotype of resistant cells. Consistent with its more specific effect on translocation, hygromycin B exhibits monophasic inhibition of polypeptide synthesis, suggesting that it binds to a single site on the ribosome.
Solubility
Fully miscible in water and also soluble in methanol or ethanol and buffer solution.
Notes
Protect from heat. Keep the container tightly closed and place it in a cool, dry and well ventilated condition. Store in freezer at -20°C.
RUO – Research Use Only