Phenol:Chloroform:Isoamyl Alcohol (25:24:1), Molecular Biology Grade, Ultrapure

Phenol:Chloroform:Isoamyl Alcohol (25:24:1), Molecular Biology Grade, Ultrapure should be stored at 4 degrees C.

We recommend the following Phenol/Chloroform RNA Isolation Method:
A. Guanidine HCI Homogenization Buffer for RNA Isolation: 8.0M Guanidine HCI, 0.1M Sodium Acetate, pH 5.5, 5.0mM Dithiothreitol, 0.5% Sodium Lauryl Sarcosinate
B. Extraction with Phenol: Most extractions are performed using equal volumes of equilibrated phenol with respect to the sample volume. Normally, the extraction is performed twice with phenol and once with a 1:1 solution of phenol/chloroform. Hence, it is estimated that 25 ml of phenol is required for every 10 ml of sample. Note: If regular phenol is purchased, it will need to be equilibrated before use. For this reason, many customers may prefer to purchase phenol from USB that is already equilibrated (Cat. No. J75829).
C. RNA Resuspension Buffer: TE, pH 7.5

Phenol:Chloroform:lsoamyl Alcohol (25:24:1) is packaged at pH 6.7, the product is accompanied by a separate Alkaline Equilibration Buffer. When ready to use add the supplemental buffer's entire contents to the Phenol:Chloroform:lsoamyl Alcohol, then mix or shake, and allow to separate. Once this is completed the product will be at a pH of 8.0.