GeneJET Plasmid Midiprep Kit

Catalog number: K0481

Thermo Scientific™  Related applications: DNA Extraction

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Description

Thermo Scientific GeneJET Plasmid Midiprep Kit, sufficient for 25 preps, is designed for large scale isolation of high quality plasmid DNA from recombinant E. coli cultures. The kit utilizes silica-based membrane technology in the form of convenient spin column. Each prep recovers up to 200 µg of high copy number plasmid DNA. The purified plasmid DNA can be used in a wide variety of molecular biology procedures, such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing, in vitro transcription, and transfection of robust cell lines.

Highlights

Efficient—high yields up to 200 µg of high quality plasmid DNA from 50 mL of bacterial culture
High concentration of DNA purified—> 400 ng/ µL
Convenient—spin column format, centrifuge or vacuum protocols
Fast—procedure takes 60 minutes, considerably faster than using gravity flow columns

Applications

• Isolated plasmid DNA is suitable for all conventional molecular biology procedures, including:
FastDigest restriction enzyme digestion
• Automated fluorescent sequencing
• PCR
• Cloning
• Transformation
In vitro transcription
• Transfection of robust cell lines
For Research Use Only. Not for use in diagnostic procedures.

Specifications

High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Sample Type (General): Bacteria
Downstream Application: Sequencing, PCR, Transfection, Real-Time Quantitative PCR (qPCR), Transformation, Nucleic Acid Labeling, In Vitro Transcription, Cloning
Product Size: 25 preps

Documents

Manuals & protocols