Thermo Scientific™

GeneJET Plasmid Midiprep Kit

Catalog number:  K0481

Related applications:

DNA Extraction

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Thermo Scientific GeneJET Plasmid Midiprep Kit, sufficient for 100 preps, is designed for large scale isolation of high quality plasmid DNA from recombinant E. coli cultures. The kit utilizes silica-based membrane technology in the form of convenient spin column. Each prep recovers up to 200 µg of high copy number plasmid DNA. The purified plasmid DNA can be used in a wide variety of molecular biology procedures, such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing, in vitro transcription, and transfection of robust cell lines.


Efficient—high yields up to 200 µg of high quality plasmid DNA from 50 mL of bacterial culture
High concentration of DNA purified—> 400 ng/ µL
Convenient—spin column format, centrifuge or vacuum protocols
Fast—procedure takes 60 minutes, considerably faster than using gravity flow columns


• Isolated plasmid DNA is suitable for all conventional molecular biology procedures, including:
FastDigest restriction enzyme digestion
• Automated fluorescent sequencing
• Cloning
• Transformation
In vitro transcription
• Transfection of robust cell lines
For Research Use Only. Not for use in diagnostic procedures.


For Use With (Application): Real-Time Quantitative PCR (qPCR), Transfection, Cloning, Sequencing, In Vitro Transcription, Transformation, Nucleic Acid Labeling, PCR
No. of Reactions: 25 Preps
Purification Target: Plasmid DNA
Sample Type: Bacteria
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Prep Scale: 100-200 µg (Medium-Scale) Plasmid DNA
Product Line: GeneJET