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DecaLabel DNA Labeling Kit

Catalog number: K0622

Thermo Scientific™  Related applications: Nucleic Acid Labeling & Oligo Synthesis

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Thermo Scientific DecaLabel DNA Labeling Kit is an advanced system for fast synthesis of radiolabeled DNA probes of high specific activity. It's based on the improved random-primed method developed by Feinberg and Vogelstein.

The primary improvement over traditional random-primer kits involves the use of random decamers instead of hexamers to ensure more efficient annealing with DNA at 37°C. In addition, Klenow Fragment, exo- has been genetically engineered to remove exonuclease activity. The improved enzyme does not degrade the labeled probe during reaction and allows high labeling yields even from low template amounts. DNA fragments of any length can be uniformly labeled. Two labeling mixes are provided for flexibility in using either radiolabeled dATP or dCTP.


High specific radioactivity of probes—greater than 1 x 109 dpm/µg DNA
Fast—5 minutes at 37°C
High yields with Klenow Fragment, exo no degradation of a labeled probe during reaction
Flexible—suitable for either radiolabeled dATP or dCTP


• Generation of radiolabeled DNA probes for use in a variety of hybridization experiments: Southern and Northern blots, colony/plaque hybridizations, dot/slot blots, and in situ hybridizations.


Klenow Fragment, exo-
• Decanucleotides in Reaction Buffer
• Mix A (-dATP)
• Mix C (-dCTP)
dNTP Mix
• Control Template
Water, nuclease-free
• Detailed Protocol

Random decamers are annealed to a denatured template DNA, and radiolabeled dNTPs are then incorporated into new DNA strands by Klenow Fragment, exo- (see Figure 1 under Supporting Data).
For Research Use Only. Not for use in diagnostic procedures.


Detection Method: Radioactive
Final Product: DNA
Includes Label or Dye: No
Labeling Method: Direct Labeling
Labeling Target: DNA (General), Genomic DNA, PCR Products
Product Line: DecaLabel
Product Size: 30 reactions


Manuals & protocols