The pcDNA™3.3-TOPO® TA Cloning® Kit is designed to deliver exceptionally high levels of transgene expression in adherent cells as well as in the FreeStyle™ MAX CHO and FreeStyle™ MAX 293 suspension cell systems. The pcDNA3.3™-TOPO® TA Cloning® Kit is supplied with the TOPO®-adapted plasmid vector, all reagents for cloning, and One Shot® TOP10 competent cells. The pcDNA™3.3-TOPO® vector offers the following benefits:
• TOPO® adapted for cloning PCR products with >85% efficiency • High-level gene expression in a wide range of mammalian cells due to the native cytomegalovirus (CMV) immediate-early promoter/enhancer sequence (672 bp) • Vector is ideal for large-scale protein expression • Generation of native protein without extraneous amino acids
The pcDNA™3.3-TOPO® vector is also included with the OptiCHO™ Antibody Express System for cloning and expression of light and heavy antibody chains.
For Research Use Only. Not for use in diagnostic procedures.
Constitutive or Inducible System
TOPO TA Cloning Kit
Selection Agent (Eukaryotic)
Protein Production, Constitutive Expression
Contents & Storage
Box 1 (store at -20°C) • 20 µl pcDNA™3.3-TOPO™ vector, TOPO™ adapted (5–10 ng/µl) • 100 µl 10X PCR Buffer • 10 µl dNTP Mix • 50 µl Salt Solution • 1 ml Sterile Water • 10 µl Control PCR Template (50 ng/µl) • 10 µl Control PCR Primers (100 ng/µl each) • 20 µl CMV Forward Sequencing Primer (100 ng/µl) • 20 µl TK polyA Reverse Sequencing Primer (100 ng/µl) • 10 µl pcDNA™3.3-TOPO™/lacZ Expression Control Plasmid (500 ng/µl)
Box 2 (store at -80°C) • 11 × 50 µl TOP10 E. coli • 50 µl pUC19 Control DNA (10 pg/µl) • 6 ml S.O.C. Medium