Invitrogen™

TaqMan™ Reverse Transcription Reagents

Catalog number:  N8080234

Related applications:

Reverse Transcription

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Description

TaqMan® Reverse Transcription Reagents provide the necessary reagents for reverse transcriptase-PCR (RT-PCR). Random hexamers, oligo (dT)16, and reverse primers are included for cDNA synthesis flexibility. Advantages of TaqMan® Reverse Transcription Reagents:

• Contain MultiScribe® Reverse Transcriptase, a recombinant moloney murine leukemia virus reverse transcriptase (MMLV RT)
• Random hexamers and oligo (dT)16 included for flexibility
• Individual components provide versatility in assay set-up
• Compatible with two-step RT-PCR reactions

Using TaqMan® Reverse Transcription Reagents
TaqMan® Reverse Transcription Reagents provide all the necessary components to perform the reverse transcription of RNA to cDNA. For two-step RT-PCR, random hexamers, oligo (dT)16, and sequence-specific reverse primers can all be used. The choice of primers for reverse transcription is best made after experimentally evaluating all three priming systems.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Final Product Type: cDNA (First-Strand)
For Use With (Application): Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR)
Optimal Reaction Temperature: 42° C
Product Line: TaqMan™
Quantity: 200 reactions
Reaction Format: SuperMix or Master Mix, Separate Components
Reverse Transcriptase: MultiScribe™
Sample Type: RNA
Shipping Condition: Dry Ice
Size (Final Product): 5 kb or less

Contents & storage

Contents:
• 1 × 100 µL of MultiScribe™ Reverse Transcriptase (50U/µL)
• 1 × 100 µL of RNase Inhibitor (20U/µL)
• 1 × 1 mL of dNTP Mixture (2.5 mM each dNTP)
• 1 × 100 µL of Oligo d(T)16 (50 µM)
• 1 × 100 µL of Random Hexamers (50 µM)
• 1 × 1.5 mL of 10X RT Buffer
• 1 × 1.5 mL of MgCl2 Solution

Reagents should be stored between -15°C and -25°C.

A note about the size: This kit contains enough reagents for 200 reverse transcriptase reactions when the reactions are set up at 10 µL each.