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Invitrogen
Phospho-SHC (Tyr349) Polyclonal Antibody
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FIGURE: 1 / 4
Phospho-SHC (Tyr349) Antibody (PA5-38073) in ICC/IF
Immunofluorescence analysis of Phospho-SHC (Tyr349) was performed using 90% confluent log phase A-431 cells treated with 200 ng/mL of EGF for 10 minutes. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Phospho-SHC (Tyr349) Rabbit Polyclonal Antibody (Product ... View More
Product Details
PA5-38073
Applications
Tested Dilution
Publications
Product Specifications
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic phosphopeptide derived from human Shc around the phosphorylation site of Tyr349 (H-Q-YP-Y-N)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
Target Information
Skp2 (S-phase Kinase-associated Protein 2) belongs to the family of F-box proteins that interact with the Cyclin A-Cdk2 complex. Skp2 is essential for the G1-S transition in both transformed cells and diploid fibroblasts. Biochemical and genetic experiments have demonstrated that Skp2 is required for the ubiquitination and consequent degradation of p27 in cultured mammalian cells and in vitro reconstitution assays. In normal tissues, Skp2 is expressed in tonsil and placenta. In tumor tissues, Skp2 is expressed widely, in some colon, prostate, pancreas, and skin cancers, with high expression in lung, breast, ovarian, and endometrial cancers. Research suggests that Skp2 plays a role in oncogenesis, particularly in the pathogenesis of lymphomas and breast cancers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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