Thermo Scientific™

IPTG, dioxane-free

Catalog number: R0393
Thermo Scientific™

IPTG, dioxane-free

Catalog number: R0393
Catalog Number
R0393
Unit Size
5 x 5 g
Price (USD)
624.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
5 g
Price (USD)
209.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
R03935 x 5 g
624.00
Your Price:
***
R03925 g
209.00
Your Price:
***
Product Overview
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Additional Information
Recommendations
Recommendations
Thermo Scientific IPTG (isopropyl-beta-D-thiogalactopyranoside) is a highly stable synthetic analog of lactose. It inactivates the lac repressor and induces synthesis of beta-galactosidase, an enzyme that promotes lactose utilization. IPTG is used to induce expression of cloned genes under control of the lac operon (see Reference 1). It is used in conjunction with X-Gal (#R0941) to determine the lac phenotype in blue/white colony screening.

Applications

• Blue/white colony screening to distinguish recombinant (white) from non-recombinant (blue) colonies (see Reference 1)
• Induction of gene expression under control of the lac promoter

Note
• Preparation of a 100 mM (23.83 mg/mL) stock solution in water is recommended
• For blue/white colony screening use 0.1 mM final IPTG concentration in LB (Luria Broth) media
• IPTG, dioxane-free, can be stored at +4°C

Related Products
IPTG Solution, ready-to-use
IPTG, dioxane-free

Specifications

Chemical Name or Material
Isopropylthiogalactoside
Concentration
0.1 mM
Format
Powder
Product Type
IPTG Solution
Quantity
25 g

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