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Determine the indole production by members of the Enterobacteriaceae using Thermo Scientific™ Remel™ Indole Broth. Indole production is an important characteristic in the identification of many species of bacteria, specifically enteric gram-negative rods. Indole Broth is recommended by the American Public Health Association (APHA) and in the Identification of Enterobacteriaceae for use in the differentiation of enteric gram-negative rods1,2.
| Description | Indole Broth (Tryptone Broth) |
| Format | 15mm x 103mm Tube |
| Product Type | Microbiology Media |
| Quantity | 20 x 4 mL |
| Unit Size | Each |
| Description | Indole Broth (Tryptone Broth) |
| Format | 15mm x 103mm Tube |
| Product Type | Microbiology Media |
| Quantity | 100 x 4 mL |
| Unit Size | Each |
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| R061172 | Each | 20 x 4 mL | Request A Quote | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Microorganisms that possess the enzyme tryptophanase have the ability to hydrolyze and deaminate tryptophan with the production of indole, pyruvic acid and ammonia3. Indole that splits from the tryptophan molecule on addition of the reagent ρ-dimethylaminobenzaldehyde results in a distinct red color that can be easily detected4.
- Ready to use - Liquid media
- Recommended by APHA in the Identification of Enterobacteriaceae for use in the differentiation of enteric gram-negative rods1,2.
- Easy to interpret -
- Positive test - Pink to red colored ring at the interface of the broth and the reagent
- Negative test - No color development
Indole Broth contains casein peptone that supplies essential growth factors, trace elements, and is the source of tryptophan in this medium.. Sodium chloride maintains osmotic equilibrium. Certain gram-negative rods release indole after hydrolyzing tryptophan. After addition of dimethylaminobenzaldehyde, it combines with the indole and produces a distinct red color.
- MacFaddin, J.F. 2000. Biochemical Tests for Identification of Medical Bacteria. 3rd Lippincott Williams & Wilkins, Philadelphia, PA.
- Koneman, E.W., S.D. Allen, W.M. Janda, P.C. Schreckenberger, and W.C. Winn, Jr. 1997. Color Atlas and Textbook of Diagnostic Microbiology. 5th Lippincott Williams & Wilkins, Philadelphia, PA.
- Eaton, A.D., L.S. Clesceri, and A.E. Greenberg. 1995. Standard Methods for the Examination of Water and Wasterwater. 19th APHA, Washington, D.C.
- Ewing, W.H. 1986. Edwards and Ewing’s Identification of 4th ed. Elsevier, New York, NY.
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