Exo-Resistant Random Primer

Catalog number: SO181

Thermo Scientific™  Related applications: Nucleic Acid Labeling & Oligo Synthesis

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Thermo Scientific Exonuclease-Resistant Random Primer is a mixture of single-stranded random oligonucleotides used for highly efficient random priming of various DNA synthesis reactions. This primer has two 3'-terminal phosphorothioate (PTO) modifications that are resistant to the 3'→5' exonuclease activity of proofreading DNA polymerases, such as Klenow Fragment and phi29 DNA Polymerase. It also has 5'- and 3'-hydroxyl ends. The Exo-Resistant Random Primer is supplied as a ready-to-use, 20X concentrated aqueous solution.


• 3’-terminal PTO modifications protect from 3’→5’ exonuclease degradation and ensure efficient priming of DNA synthesis


• Strand displacement amplification of genomic DNA (see​ Reference 2), plasmids and phage DNA
• DNA labeling by random priming (see​ References 4-6)

• Use of this enzyme in certain applications may be covered by patents and may require a license.
For Research Use Only. Not for use in diagnostic procedures.


Primer Type: Random Primers
Product Size: 100 µL


Manuals & protocols