Sortase A localizes to distinct foci on the Streptococcus pyogenes membrane.
AuthorsRaz A, Fischetti VA,
JournalProc Natl Acad Sci U S A
PubMed ID19017791
'Cell wall peptidoglycan-anchored surface proteins are essential virulence factors in many gram-positive bacteria. The attachment of these proteins to the peptidoglycan is achieved through a transpeptidation reaction, whereby sortase cleaves a conserved C-terminal LPXTG motif and covalently attaches the protein to the peptidoglycan precursor lipid II. It is unclear how ... More
Polar localization of virulence-related Esx-1 secretion in mycobacteria.
AuthorsCarlsson F, Joshi SA, Rangell L, Brown EJ,
JournalPLoS Pathog
PubMed ID19180234
'The Esx-1 (type VII) secretion system is critical for virulence of both Mycobacterium tuberculosis and Mycobacterium marinum, and is highly conserved between the two species. Despite its importance, there has been no direct visualization of Esx-1 secretion until now. In M. marinum, we show that secretion of Mh3864, a novel ... More
Use of confocal microscopy to analyze the rate of vancomycin penetration through Staphylococcus aureus biofilms.
AuthorsJefferson KK, Goldmann DA, Pier GB
JournalAntimicrob Agents Chemother
PubMed ID15917548
'When bacteria assume the biofilm mode of growth, they can tolerate levels of antimicrobial agents 10 to 1,000 times higher than the MICs of genetically equivalent planktonic bacteria. The properties of biofilms that give rise to antibiotic resistance are only partially understood. Inhibition of antibiotic penetration into the biofilm may ... More
Imaging peptidoglycan biosynthesis in Bacillus subtilis with fluorescent antibiotics.
AuthorsTiyanont K, Doan T, Lazarus MB, Fang X, Rudner DZ, Walker S
JournalProc Natl Acad Sci U S A
PubMed ID16832063
'The peptidoglycan (PG) layers surrounding bacterial cells play an important role in determining cell shape. The machinery controlling when and where new PG is made is not understood, but is proposed to involve interactions between bacterial actin homologs such as Mbl, which forms helical cables within cells, and extracellular multiprotein ... More
Fluorescence ratio imaging microscopy shows decreased access of vancomycin to cell wall synthetic sites in vancomycin-resistant Staphylococcus aureus.
AuthorsPereira PM, Filipe SR, Tomasz A, Pinho MG,
JournalAntimicrob Agents Chemother
PubMed ID17646417
'A new method of fluorescence ratio imaging microscopy was used to compare the in vivo binding capacity and the access of a fluorescent derivative of vancomycin to the cell wall synthetic sites in isogenic pairs of vancomycin-susceptible and -resistant laboratory mutants and vancomycin-intermediate and -susceptible clinical isolates of Staphylococcus aureus. ... More
Activities of antistaphylococcal antibiotics towards the extracellular and intraphagocytic forms of Staphylococcus aureus isolates from a patient with persistent bacteraemia and endocarditis.
Decreased susceptibility of Staphylococcus aureus to antistaphylococcal agents may be associated with inability to eradicate intracellular forms, which could explain therapeutic failures. This hypothesis was tested using clinical isolates obtained from a patient with persistent staphylococcal bacteraemia under therapy. Four isogenic isolates (three from tissue, one from blood) with increased ... More
Distribution of protein A on the surface of Staphylococcus aureus.
AuthorsDeDent AC, McAdow M, Schneewind O,
JournalJ Bacteriol
PubMed ID17416657
Surface proteins of Staphylococcus aureus fulfill many important roles during the pathogenesis of human infections and are anchored to the cell wall envelope by sortases. Although the chemical linkage of proteins to cell wall cross bridges is known, the mechanisms whereby polypeptides are distributed on the staphylococcal surface have not ... More
An association between peptidoglycan synthesis and organization of the Streptococcus pyogenes ExPortal.
Authors
JournalMBio
PubMed ID24065630
Staphylococcus aureus ST228 and ST239 as models for expression studies of diverse markers during osteoblast infection and persistence.
Authors
JournalMicrobiologyopen
PubMed ID33970534
Regulated shift from helical to polar localization of Listeria monocytogenes cell wall-anchored proteins.
Authors
JournalJ Bacteriol
PubMed ID21725001
Mycelium differentiation and antibiotic production in submerged cultures of Streptomyces coelicolor.
Authors
JournalAppl Environ Microbiol
PubMed ID18441105
Mechanism of polarization of Listeria monocytogenes surface protein ActA.
Authors
JournalMol Microbiol
PubMed ID16430699
Detection of methicillin-resistant Staphylococcus aureus persistence in osteoblasts using imaging flow cytometry.
Authors
JournalMicrobiologyopen
PubMed ID32237200
Characterization of SCO4439, a D-alanyl-D-alanine carboxypeptidase involved in spore cell wall maturation, resistance, and germination in Streptomyces coelicolor.
Authors
JournalSci Rep
PubMed ID26867711
Cationic antimicrobial peptides disrupt the Streptococcus pyogenes ExPortal.
Authors
JournalMol Microbiol
PubMed ID22780862
Assessment of Gram- and Viability-Staining Methods for Quantifying Bacterial Community Dynamics Using Flow Cytometry.
Authors
JournalFront Microbiol
PubMed ID32676069
Subcompartmentalization by cross-membranes during early growth of Streptomyces hyphae.