Invitrogen™

Mitochondrial Membrane Potential Apoptosis Kit, with Mitotracker™ Red & Annexin V Alexa Fluor™ 488, for flow cytometry

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Description

This flow cytometry product provides a rapid and convenient assay for two hallmarks of apoptosis - phosphatidylserine externalization and changes in mitochondrial membrane potential. After staining a cell population with Alexa Fluor™ 488 annexin V and MitoTracker™ Red CMXRos dye in the provided binding buffer, live cells exhibit very little green fluorescence and bright red fluorescence, whereas apoptotic cells exhibit green fluorescence and decreased red fluorescence. These populations can easily be distinguished using a flow cytometer, and the 488 nm line of an argon-ion laser can be used to excite both dyes.

View a selection guide for all apoptosis assays for flow cytometry.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Excitation/Emission: Mitotracker™ red 579⁄599, Alexa Fluor® 488: 499⁄521
Flow Cytometer Laser Lines: 488
Label Type: Alexa Fluor Dyes, Other Label(s) or Dye(s)
Label or Dye: Alexa Fluor™ 488, MitoTracker® Red
No. of Reactions: 50
Product Line: Alexa Fluor™, MitoTracker®
Shipping Condition: Wet Ice
Detection Method: Fluorescent
For Use With (Equipment): Fluorescence Microscope, Flow Cytometer
Format: Tube(s), Slide(s)

Contents & storage

Contains 1 vial of annexin, Alexa Fluor® 488 conjugate (250 µL), 3 vials of MitoTracker® red (50 µg per vial), 1 bottle of annexin binding buffer (5X solution, 15 mL), and 1 vial of DMSO (100 µL).

Store in refrigerator (2–8°C) and protect from light.