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      Invitrogen™

      Mitochondrial Membrane Potential Apoptosis Kit, with Mitotracker™ Red & Annexin V Alexa Fluor™ 488, for flow cytometry

      Mitochondrial Membrane Potential Apoptosis Kit, with Mitotracker™ Red & Annexin V Alexa Fluor™ 488, for flow cytometry

      Catalog number:  V35116

       Related applications: Cell Viability, Proliferation & Function | Cellular Imaging | Flow Cytometry | Pharma & Biopharma | Target-Based ADME⁄Tox Assays

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      Description

      This flow cytometry product provides a rapid and convenient assay for two hallmarks of apoptosis - phosphatidylserine externalization and changes in mitochondrial membrane potential. After staining a cell population with Alexa Fluor™ 488 annexin V and MitoTracker™ Red CMXRos dye in the provided binding buffer, live cells exhibit very little green fluorescence and bright red fluorescence, whereas apoptotic cells exhibit green fluorescence and decreased red fluorescence. These populations can easily be distinguished using a flow cytometer, and the 488 nm line of an argon-ion laser can be used to excite both dyes.

      View a selection guide for all apoptosis assays for flow cytometry.
      For Research Use Only. Not for use in diagnostic procedures.

      Specifications

      Flow Cytometer Laser Lines: 488
      Detection Method: Fluorescent
      Excitation⁄Emission (nm): Alexa Fluor® 488: 499⁄521, Mitotracker™ red 579⁄599
      For Use With (Equipment): Flow Cytometer, Fluorescence Microscope
      Format: Slide(s), Tube(s)
      Label or Dye: Alexa Fluor™ 488, MitoTracker® Red
      Number of Reactions: 50
      Product Line: Alexa Fluor™, MitoTracker®
      Product Size: 1 kit
      Shipping Condition: Wet Ice

      Contents & storage

      Contains 1 vial of annexin, Alexa Fluor® 488 conjugate (250 µL), 3 vials of MitoTracker® red (50 µg per vial), 1 bottle of annexin binding buffer (5X solution, 15 mL), and 1 vial of DMSO (100 µL).

      Store in refrigerator (2–8°C) and protect from light.

      Documents

      Manuals & protocols