Zenon™ pHrodo™ iFL IgG Labeling Reagents
New Zenon pHrodo Deep Red Labeling Reagents are superior in three ways: improved bioconjugation for brighter signal, redesigned protein for robust screening regardless of IgG isotype, and a new, more sensitive fluorophore.
Zenon™ pHrodo™ iFL IgG Labeling Reagents
Invitrogen™

Zenon™ pHrodo™ iFL IgG Labeling Reagents

Zenon™ pHrodo™ iFL Green-labeled or Red-labeled Fab fragments (i.e. labeling reagent) are directed against the Fc portion of an intact IgG primary antibody to form a labeling complex.
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Catalog NumberSpeciesLabel or DyeColorQuantity
Z25609MousepHrodo iFL GreenGreen250 μL
Z25618HumanpHrodo Deep RedDeep Red250 μL
Z25610MousepHrodo iFL RedRed250 μL
Z25622MousepHrodo Deep RedDeep Red250 μL
Z25611HumanpHrodo iFL GreenGreen250 μL
Z25612HumanpHrodo iFL RedRed250 μL
Z25613HumanpHrodo iFL GreenGreen1.2 mL
Z25614HumanpHrodo iFL RedRed1.2 mL
8 Options
Catalog number Z25609
Price (USD)/ 250 μL
1,200.00
-
Add to cart
Species:
Mouse
Label or Dye:
pHrodo iFL Green
Color:
Green
Quantity:
250 μL
Price (USD)/ 250 μL
1,200.00
Add to cart
Zenon™ pHrodo™ iFL IgG Labeling Reagents
Catalog numberZ25609
Price (USD)/ 250 μL
1,200.00
-
Add to cart
Streamline antibody-drug conjugate screening campaigns with Invitrogen Zenon pHrodo IgG Labeling Reagents to screen for antibody internalization. These reagents provide rapid, scalable antibody labeling and give a bright fluorescence-based indication of antibody uptake and trafficking in target cells when analyzed by flow cytometry, fluorescence microscopy, or high-content screening.

Zenon™ pHrodo™ iFL Green-labeled or Red-labeled Fab fragments (i.e. labeling reagent) are directed against the Fc portion of an intact IgG primary antibody to form a labeling complex. Formation of the Fab–antibody complex occurs in fewer than 5 minutes. Because the pHrodo™ iFL dyes dramatically increase in fluorescence as the pH of their surroundings becomes more acidic, the antibodies coupled with the Zenon™ pHrodo™ iFL IgG Labeling Reagents provide an excellent measure of endocytic activity based on the acidification of the labeled antibodies as they are ingested by the cell.

The Zenon™ pHrodo™ iFL IgG Labeling Reagents contain sufficient material to label one to four 96-well plates, depending on the Zenon™ labeling reagent concentration used. Only a small amount of starting material is needed, and the method is optimized for efficient labeling of antibodies in serum, ascites fluid, or hybridoma suspensions.

Other advantages of the pH-sensitive Zenon™ pHrodo™ iFL IgG Labeling Reagents include:

  • Simplicity—No reagent purification is required
  • Accuracy—No wash steps or quenchers are needed, minimizing cross-reactivity or background
  • Speed—Labeled antibodies are typically ready to use in 10 minutes
  • Specificity—Dyes are designed for the specific detection of phagocytosis and endocytosis

Preserve primary antibody function and affinities

Reactive dye labeling of primary antibodies can have unpredictable and undesirable outcomes. One of these is reduced binding affinity due to label addition in the binding pocket. The Zenon™ antibody labeling approach, which is targeted to the Fc tail, resolves this potential issue.

Try custom antibody conjugates

If the antibody conjugate you need is not available as one of our stocked products, we will prepare a custom antibody conjugate for you. Our custom conjugation service is efficient and confidential, and we stand by the quality of our work. We are ISO 13485:2000 certified.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
DescriptionpHrodo™ iFL Green, 250 μL
Kit Contents1 vial of Zenon™ pHrodo™ iFL Green mouse labeling reagent (250 μL)
Product TypeLabeling Reagent
Quantity250 μL
Excitation/Emission505/530 nm
Product LineZenon™
Label or DyepHrodo iFL Green
ColorGreen
Labeling TargetIgG
SpeciesMouse
Label TypepHrodo™ (pH sensitive)
Unit Size250 μL
Contents & Storage
Store in refrigerator (2°C to 8°C) and protect from light