Invitrogen™

Zenon™ pHrodo™ iFL Red Human IgG Labeling Reagent

Catalog number:  Z25612

 Related applications:

Antibody Labeling

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Description

Zenon labeling technology provides a fast, versatile, and reliable method for adding a fluorescent label to an antibody. Only a small amount of starting material is needed, and the method is optimized for efficient labeling of antibodies in serum, ascites fluid, or hybridoma suspensions.

Combined with pHrodo iFL dye, this technology provides a rapid, scalable, and easy way to determine how well an antibody internalizes into target cells. pHrodo iFL Red dye dramatically increases its fluorescence as the pH of its surroundings becomes more acidic.

• Labeled antibodies typically ready to use in 10 minutes
• Simple, no purification required
• Fast, accurate internalization results—no need for wash steps or quenchers

Preserve primary antibody function and affinities
Reactive dye labeling of primary antibodies can have unpredictable and undesirable outcomes. One of these is reduced binding affinity due to label addition in the binding pocket. The Zenon antibody labeling approach, which is targeted to the Fc tail, avoids this outcome.

Custom antibody conjugates
If the antibody conjugate you need is not available as one of our stocked products, we will prepare a custom antibody conjugate for you. Our custom conjugation service is efficient and confidential, and we stand by the quality of our work. We are ISO 9001:2000 certified.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Excitation⁄Emission (nm): 560⁄585
Label or Dye: pHrodo&trad;e iFL Red
Labeling Scale: 75 µg
Labeling Target: Antibodies (IgG)
Product Line: Zenon™ , pHrodo™
Product Size: 240 µL
Shipping Condition: Room Temperature

Contents & storage

Store in refrigerator at 2–8°C.

Documents

Manuals & protocols