Fetal Bovine Serum, qualified, Australia
Fetal Bovine Serum, qualified, Australia
Citations & References (1)
Gibco™

Fetal Bovine Serum, qualified, Australia

Gibco™のウシ胎児血清は、基礎細胞培養、特殊研究、および特異的アッセイに優れた価値を提供し、研究ニーズや予算要件を満たすことができる、一貫した品質と受賞歴のあるサポートにより、研究者の信頼を得ることができます血清カテゴリー：プレミアム（セキュア）原産国：オーストラリア。エンドトキシン濃度：≤5詳細を見る
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製品番号（カタログ番号）数量フォーマット純度または品質グレード
10099141500 mLボトル適格
A316100150 mLOne Shot適格
Premium Plus Fetal Bovine Serum (FBS)は安定性や製造方法を維持したまま、仕様を強化しました。
製品番号（カタログ番号） 10099141
現在の製品を選択する

Gibco™のウシ胎児血清は、基礎細胞培養、特殊研究、および特異的アッセイに優れた価値を提供し、研究ニーズや予算要件を満たすことができる、一貫した品質と受賞歴のあるサポートにより、研究者の信頼を得ることができます

血清カテゴリー：プレミアム（セキュア）

原産国：オーストラリア。

エンドトキシン濃度：≤5 EU/mL

ヘモグロビン濃度：≤30 mg/dL（通常濃度≤25 mg/dL）。

For research use or further manufacturing use only. Serum and blood proteins are not for direct administration into humans or animals.
仕様
エンドトキシン濃度≤5 EU/mL
ヘモグロビン濃度≤30 mg/dL
純度または品質グレード適格
出荷条件凍結
種畜牛/ウシ
Age胎児
原産国オーストラリア
形状液体
フォーマットボトル
製品タイプウシ胎児血清
数量500 mL
Serum Treatment標準（滅菌ろ過済み）
無菌性滅菌済
Unit SizeEach
組成および保存条件
保存条件：≤-10°C
輸送条件: 凍結

よくあるご質問（FAQ）

What are the benefits and disadvantages of using heat-inactivated FBS in cell culture?

  1. Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat-inactivated serum is recommended include immunological studies and culturing of embryonic stem cells (ESCs), insect cells, and smooth muscle cells.
  2. Heat inactivation helps to achieve bottle-to-bottle and lot-to-lot stability by neutralizing many factors that can vary largely from lot to lot.
  3. There aren't necessarily disadvantages to heat inactivation of FBS, but there is some evidence that suggests there may be no added benefit to it unless you are carrying out immune studies.

Note: Heat inactivation is performed in a 56 degrees C water bath for 30 min with swirling every 10 min or so for heat distribution and to lower the degree of protein aggregation/flocculant precipitation. Note: If the time or temperature is exceeded, the serum may thicken to a gel. If this occurs, the serum is no longer usable. Unnecessary heat inactivation can take up time and potentially lead to wasted reagents if a mistake is made during the protocol1.

1. Pellerin, et al., Bioengineering, published in 2021.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How long can I store Gibco Fetal Bovine Serum at 4 degrees C?

This product can be stored at 4 degrees C for up to 4 weeks. Once the medium has been supplemented with serum, we recommend using it within 2-4 weeks.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How should I thaw Gibco Fetal Bovine Serum?

We recommend thawing the serum overnight at 4 degrees C or in a 37 degrees C water bath, removing as soon as it is thawed. Once thawed, aliquot into single-use sizes and freeze the aliquots. Each aliquot should ideally be thawed only one additional time as repeated freeze-thaw cycles are not recommended.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How can I minimize lot-to-lot variability in serum?

You can test each new lot to be sure that it works for your specific applications. Alternatively, our serum sales team can determine the most consistent, highest-performing serum lot available using the Gibco iMatch Sera Lot Matching Tool.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I aliquot and refreeze serum?

Yes, after the overnight thaw, you can aliquot the serum into smaller, single-use aliquots and refreeze it.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Fetal Bovine Serum, qualified, Australia FAQs

引用および参考文献 (1)

引用および参考文献
Abstract
Lack of Fucose on Human IgG1 N-Linked Oligosaccharide Improves Binding to Human Fcgamma RIII and Antibody-dependent Cellular Toxicity.
Authors: Shields Robert L; Lai Jadine; Keck Rodney; O'Connell Lori Y; Hong Kyu; Meng Y Gloria; Weikert Stefanie H A; Presta Leonard G;
Journal:J Biol Chem
PubMed ID:11986321
Lec13 cells, a variant Chinese hamster ovary cell line, were used to produce human IgG1 that were deficient in fucose attached to the Asn(297)-linked carbohydrate but were otherwise similar to that found in IgG1 produced in normal Chinese hamster ovary cell lines and from human serum. Lack of fucose on ... More