MEM, NEAA, no glutamine
MEM, NEAA, no glutamine
Citations & References (1)
Gibco™

MEM, NEAA, no glutamine

Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used詳細を見る
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製品番号（カタログ番号）数量
1037008810 x 500 mL
10370021500 mL
製品番号（カタログ番号） 10370088
価格（JPY）
29,000
Each
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数量:
10 x 500 mL
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Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. We offer a variety of Gibco™ MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.


This MEM is modified as follows:
WithWithout
• NEAA• L-glutamine
• Phenol Red• HEPES


The complete formulation is available.

Gibco™ MEM, developed by Harry Eagle, was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow’s MEM, MEM α, DMEM, and Temin’s Modification. MEM is available with Earle’s salts for use in a CO2 incubator, or with Hanks' salts for use without CO2. This product is made with Earle’s salts.

MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
仕様
細胞株HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts
細胞タイプPrimary Rat Astrocytes
濃度1 X
製造品質cGMP-compliant under the ISO 13485 standard
製品ラインGibco
製品タイプMEM (Minimum Essential Medium)
数量10 x 500 mL
品質保持期間24 Months From Date of Manufacture
出荷条件Room Temperature
分類Animal Origin-free
Serum LevelStandard Serum Supplementation
無菌性Sterile-filtered
Sterilization MethodSterile-filtered
添加剤ありLow Glucose, Phenol Red, Non Essential Amino Acids (NEAA)
添加剤なしNo Glutamine, No HEPES, No Sodium Pyruvate
Unit SizeEach
組成および保存条件
Storage conditions: 2°C to 8°C (protect from light)
Shipping conditions: Ambient
Shelf life: 24 months from date of manufacture

よくあるご質問（FAQ）

Where can I find the osmolality for MEM Medium?

The osmolality is listed in the COA for the particular lot number of the medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How can I remove mycoplasma contamination from my cell culture medium?

Very often mycoplasma contamination cannot be removed from the culture so it should be discarded. You may have a unique culture that you prefer not to discard and would like to try to clean it. Ciprofloxacin and Plasmocin have reportedly been used for this application. If interested in a protocol or directions for use, check with the antibiotic supplier or published literature. Note that mycoplasma are very difficult to remove from culture and spread easily so the treated cultures should be quarantined until clear of mycoplasma, and your laboratory should be thoroughly cleaned.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I see a decrease in growth of my culture. What should I do?

Try changing the medium or serum. Compare media formulations for differences in glucose, amino acids, and other components. Compare an old lot of serum with a new lot. Increase initial cell inoculums. Lastly, adapt cells sequentially to new medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all MEM, NEAA, no glutamine, 10 x 500 mL FAQs

引用および参考文献 (1)

引用および参考文献
Abstract
Monodansylpentane as a blue-fluorescent lipid-droplet marker for multi-color live-cell imaging.
Authors:Yang HJ, Hsu CL, Yang JY, Yang WY
Journal:PLoS One
PubMed ID:22396789
Lipid droplets (LDs) are dynamic cellular organelles responsible for the storage of neutral lipids, and are associated with a multitude of metabolic syndromes. Here we report monodansylpentane (MDH) as a high contrast blue-fluorescent marker for LDs. The unique spectral properties make MDH easily combinable with other green and red fluorescent ... More