MCDB 131 Medium, no glutamine

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Citations & References (1)

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MCDB 131 Medium, no glutamine

MCDB 131 Medium was originally developed by Knedler and Ham as reduced serum-supplemented medium for the culture of human microvascular詳細を見る

製品番号（カタログ番号）	数量
10372019	500 mL

製品番号（カタログ番号） 10372019

価格（JPY）

5,700

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500 mL

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MCDB 131 Medium was originally developed by Knedler and Ham as reduced serum-supplemented medium for the culture of human microvascular endothelial cells (HMVEC). MCDB 131 Medium is also used with other cell types, including human omental microvascular cells, hepatocytes, myocytes, and smooth muscle cells.

This MCDB 131 Medium is manufactured as follows:

With	Without
• Phenol Red	• L-glutamine
• Sodium pyruvate	• HEPES

The complete formulation is available.

Using MCDB 131
MCDB 131 Medium contains many components not found in traditional basal media, such as trace elements, putrescine, adenine, thymidine, and higher levels of some amino acids and vitamins. These additions allow the medium to be supplemented with very low levels of serum or defined components. MCDB 131 Medium contains no proteins or growth factors, and is often supplemented with EGF, hydrocortisone, glutamine, and low levels of Fetal Bovine Serum (FBS). The FBS concentration must be optimized for each cell type. MCDB 131 Medium uses a sodium bicarbonate buffer system (1.176 g/L), and therefore requires a 5–10% CO₂ environment to maintain physiological pH.

Dual-site cGMP manufacturing
For supply chain continuity, we manufacture MCDB 131 at two separate facilities, located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP-manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers.

研究用途にのみご使用ください。診断目的には使用できません。

細胞タイプHuman Microvascular Endothelial Cells (HMVEC), Human Omental Microvascular Cells, Hepatocytes, Myocytes, Smooth Muscle Cells

濃度1 X

製品ラインGibco

製品タイプMCDB 131 Medium

数量500 mL

品質保持期間12 Months From Date of Manufacture

出荷条件Room Temperature

Culture TypeMammalian Cell Culture

形状Liquid

無菌性Sterile-filtered

添加剤ありLow Glucose, Phenol Red, Sodium Pyruvate

添加剤なしNo Glutamine, No HEPES

Unit SizeEach

組成および保存条件

Storage conditions: 2–8°C (protect from light)
Shelf life: 12 months from date of manufacture

よくあるご質問（FAQ）

What does MCDB stand for?

MCDB stands for Molecular, Cellular and Developmental Biology Department at the University of Colorado. Researchers in this department developed the MCDB media series, of which MCDB 131 is commonly used.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How can I remove mycoplasma contamination from my cell culture medium?

Very often mycoplasma contamination cannot be removed from the culture so it should be discarded. You may have a unique culture that you prefer not to discard and would like to try to clean it. Ciprofloxacin and Plasmocin have reportedly been used for this application. If interested in a protocol or directions for use, check with the antibiotic supplier or published literature. Note that mycoplasma are very difficult to remove from culture and spread easily so the treated cultures should be quarantined until clear of mycoplasma, and your laboratory should be thoroughly cleaned.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I see a decrease in growth of my culture. What should I do?

Try changing the medium or serum. Compare media formulations for differences in glucose, amino acids, and other components. Compare an old lot of serum with a new lot. Increase initial cell inoculums. Lastly, adapt cells sequentially to new medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all MCDB 131 Medium, no glutamine FAQs

引用および参考文献 (1)

引用および参考文献

Abstract

Directed differentiation of pancreatic δ cells from human pluripotent stem cells.

Authors:Chen L,Wang N,Zhang T,Zhang F,Zhang W,Meng H,Chen J,Liao Z,Xu X,Ma Z,Xu T,Liu H

Journal:Nature communications

PubMed ID:39068220

Dysfunction of pancreatic Î´ cells contributes to the etiology of diabetes. Despite their important role, human Î´ cells are scarce, limiting physiological studies and drug discovery targeting Î´ cells. To date, no directed Î´-cell differentiation method has been established. Here, we demonstrate that fibroblast growth factor (FGF) 7 promotes pancreatic ... More