Invitrogen TrackIt 100 bp DNAラダーはすぐに使用可能で、100 bp~2,000 bpの範囲で二本鎖DNAのサイズ決定とおおよその定量を行えるように設計されています。100 bp DNAラダーは、クロマトグラフィーで精製された13個のDNA断片で構成されており、2000、1500、および600 bpの基準バンドを有しているため、簡単に配向できます。
TrackIt 100 bp DNAラダーは、1–2%アガロースゲルでの分離に最適です。
TrackIt 100 bp DNAラダーの特長: •鋭利で透明なバンド—クロマトグラフィー精製断片により、一貫性と信頼性の高い結果を提供。また、室温で最大6カ月間安定 • すぐに使用可能—DNAの移動を追跡する6X TrackItシアン/オレンジローディングバッファーと事前に混合 • 正確—各バンドで正確な量のDNA
• 500 μL TrackIt 100 bp DNA Ladder • 1 mL 6X TrackIt Cyan/Orange Loading Buffer
Store at room temperature or at 4°C for up to 24 months. For longer periods, store at -20°C.
よくあるご質問(FAQ)
Can I know the sequences of Invitrogen DNA ladders?
Sequences of Invitrogen DNA and RNA ladders are proprietary.
Are Invitrogen DNA ladders composed of linear or circular/supercoiled DNA?
Invitrogen DNA ladders contain linear dsDNA fragments.
Are Invitrogen DNA ladders composed of single-stranded or double-stranded DNA fragments?
Invitrogen DNA ladders are composed of double-stranded DNA fragments only.
I'm seeing anomalous migration of my DNA ladder. What happened?
This can happen if the marker was heated. Please ensure that the ladders are not heated before use.
What is the difference between the TrackIt DNA Ladders and other DNA ladders?
TrackIt DNA Ladders contain two tracking dyes in the sample buffers, which serve as visual markers for tracking electrophoresis progress through the gel, and also to indicate when maximum resolution is achieved. The tracking dyes should not obscure your visualization of DNA bands in the ladder, as the dyes run outside the limits of most DNA bands in the ladder.
TrackIt Cyan/Orange Loading Buffer is formulated with unique tracking dyes, Xylene Cyanol FF and Orange G. We recommend TrackIt Cyan/Orange Loading Buffer for DNA fragments between 10 bp and 1 kb.
The TrackIt Cyan/Yellow Loading Buffer is formulated with unique tracking dyes, Xylene Cyanol FF and Tartrazine. We recommend TrackIt Cyan/Yellow Loading Buffer for DNA fragments between 100 bp and 10 kb. The molecular weights are Xylene Cyanol FF, 638.6; Orange G, 452.4; Tartrazine, 534.4.
Note: The TrackIt DNA Ladders are not recommended for use with polyacrylamide gels and are not designed for quantitation.
Characterization of genes encoding for acquired bacitracin resistance in Clostridium perfringens.
Authors:Charlebois A, Jalbert LA, Harel J, Masson L, Archambault M
Journal:PLoS One
PubMed ID:22970221
'Phenotypic bacitracin resistance has been reported in Clostridium perfringens. However, the genes responsible for the resistance have not yet been characterized. Ninety-nine C. perfringens isolates recovered from broilers and turkeys were tested for phenotypic bacitracin resistance. Bacitracin MIC(90) (>256 µg/ml) was identical for both turkey and chicken isolates; whereas MIC(50) ... More
Detection of murine leukemia virus in the Epstein-Barr virus-positive human B-cell line JY, using a computational RNA-Seq-based exogenous agent detection pipeline, PARSES.
Authors:Lin Z, Puetter A, Coco J, Xu G, Strong MJ, Wang X, Fewell C, Baddoo M, Taylor C, Flemington EK
Journal:J Virol
PubMed ID:22238296
'Many cell lines commonly used for biological studies have been found to harbor exogenous agents such as the human tumor viruses Epstein-Barr virus (EBV) and human papillomavirus. Nevertheless, broad-based, unbiased approaches to globally assess the presence of ectopic organisms within cell model systems have not previously been available. We reasoned ... More
Antibiotic-resistant Enterococcus faecalis in abattoir pigs and plasmid colocalization and cotransfer of tet(M) and erm(B) genes.
Authors:Tremblay CL, Letellier A, Quessy S, Daignault D, Archambault M
Journal:J Food Prot
PubMed ID:22947466
'This study was conducted to determine plasmid colocalization and transferability of both erm(B) and tet(M) genes in Enterococcus faecalis isolates from abattoir pigs in Canada. A total of 124 E. faecalis isolates from cecal contents of abattoir pigs were examined for antibiotic susceptibility. High percentages of resistance to macrolides and ... More
Rescue of recent virulent and avirulent field strains of bluetongue virus by reverse genetics.
Authors:van Gennip RG, van de Water SG, Potgieter CA, Wright IM, Veldman D, van Rijn PA
Journal:PLoS One
PubMed ID:22363444
'Since 1998, Bluetongue virus (BTV)-serotypes 1, 2, 4, 9, and 16 have invaded European countries around the Mediterranean Basin. In 2006, a huge BT-outbreak started after incursion of BTV-serotype 8 (BTV8) in North-Western Europe. More recently, BTV6 and BTV11 were reported in North-Western Europe in 2008. These latter strains are ... More
TGF-a/HA complex promotes tympanic membrane keratinocyte migration and proliferation via ErbB1 receptor.
Authors:Mei Teh B, Redmond SL, Shen Y, Atlas MD, Marano RJ, Dilley RJ
Journal:Exp Cell Res
PubMed ID:23384599
'Tympanic membrane perforations are common and represent a management challenge to clinicians. Current treatments for chronic perforations involve a graft surgery and require general anaesthesia, including associated costs and morbidities. Bioactive molecules (e.g. growth factors, cytokines) play an important role in promoting TM wound healing following perforation and the use ... More