Is the Terminal Deoxynucleotidyl Transferase (TdT) within the Pierce Biotin 3' End DNA Labeling Kit (Cat. No. 89818) available separately?
Yes, but not the specific one from that same kit. We have the following comparable Terminal Deoxynucleotidyl Transferases (Cat. No. 10533065, 10533073 or EP0161, EP0162).
'Human nuclear DNA topoisomerase I (top1) plays a crucial role in DNA replication, transcription, and chromosome condensation. In this study, we show that intra- and intermolecular guanosine quartets (G-quartets) can inhibit top1-mediated DNA cleavage at a high affinity site. Top1-mediated DNA cleavage was also inhibited by a 16-mer single-stranded oligodeoxynucleotide ... More
The novel WD-repeat protein MORG1 acts as a molecular scaffold for HIF prolyl-hydroxylase 3 (PHD3).
Authors:Hopfer U, Hopfer H, Jablonski K, Stahl RA, Wolf G,
Journal:J Biol Chem
PubMed ID:16407229
'Hypoxia-inducible factor-1 (HIF-1), a transcriptional complex composed of an oxygen-sensitive alpha- and a beta-subunit, plays a pivotal role in cellular adaptation to low oxygen availability. Under normoxia, the alpha-subunit of HIF-1 is hydroxylated by a family of prolyl hydroxylases (PHDs) and consequently targeted for proteasomal degradation. Three different prolyl hydroxylases ... More
Coordinated transcription of key pathways in the mouse by the circadian clock.
Authors: Panda Satchidananda; Antoch Marina P; Miller Brooke H; Su Andrew I; Schook Andrew B; Straume Marty; Schultz Peter G; Kay Steve A; Takahashi Joseph S; Hogenesch John B;
Journal:Cell
PubMed ID:12015981
'In mammals, circadian control of physiology and behavior is driven by a master pacemaker located in the suprachiasmatic nuclei (SCN) of the hypothalamus. We have used gene expression profiling to identify cycling transcripts in the SCN and in the liver. Our analysis revealed approximately 650 cycling transcripts and showed that ... More
DEMETER, a DNA glycosylase domain protein, is required for endosperm gene imprinting and seed viability in arabidopsis.
Authors: Choi Yeonhee; Gehring Mary; Johnson Lianna; Hannon Mike; Harada John J; Goldberg Robert B; Jacobsen Steven E; Fischer Robert L;
Journal:Cell
PubMed ID:12150995
'We isolated mutations in Arabidopsis to understand how the female gametophyte controls embryo and endosperm development. For the DEMETER (DME) gene, seed viability depends only on the maternal allele. DME encodes a large protein with DNA glycosylase and nuclear localization domains. DME is expressed primarily in the central cell of ... More
The orphan nuclear receptor REV-ERBalpha controls circadian transcription within the positive limb of the mammalian circadian oscillator.
Mammalian circadian rhythms are generated by a feedback loop in which BMAL1 and CLOCK, players of the positive limb, activate transcription of the cryptochrome and period genes, components of the negative limb. Bmal1 and Per transcription cycles display nearly opposite phases and are thus governed by different mechanisms. Here, we ... More