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Citations & References (5)
Gibco™
DMEM/F-12, GlutaMAX™ supplement
DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different詳細を見る
製品番号（カタログ番号） 10565018
価格（JPY）お問い合わせください ›
5,200
Each
数量:
500 mL
DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of DMEM/F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This DMEM / F-12 is modified as follows:
| With | Without |
| • GlutaMAX | • HEPES |
| • Phenol Red |
The complete formulation is available.
Using DMEM/F-12
DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids, and vitamins with F-12's wide variety of components. DMEM/F-12 with GlutaMAX supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format. DMEM/F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM/F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO2 environment to maintain physiological pH.
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
仕様
細胞株MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts
濃度1 X
製造品質cGMP-compliant under the ISO 13485 standard
製品ラインGibco, GlutaMAX
製品タイプDMEM (Dulbecco's Modified Eagle Medium)/F-12
数量500 mL
品質保持期間12 Months From Date of Manufacture
出荷条件Room Temperature
分類Animal Origin-free
形状Liquid
Serum LevelStandard Serum Supplementation
無菌性Sterile-filtered
Sterilization MethodSterile-filtered
添加剤ありHigh Glucose, GlutaMAX, Phenol Red, Sodium Pyruvate
添加剤なしNo HEPES
Unit SizeEach
組成および保存条件
Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
よくあるご質問（FAQ）
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引用および参考文献 (5)
引用および参考文献
Abstract
Glycine-extended progastrin processing intermediates induce H+,K(+)-ATPase alpha-subunit gene expression through a novel receptor.
Journal:J Biol Chem
PubMed ID:7744746
'Although plasma levels of G-Gly are equivalent to those of gastrin, G-Gly has essentially no acute effect on gastric acid secretion. However, we have observed that inhibition of gastrin amidation leads to increased plasma concentrations of G-Gly and enhanced gastric acid secretion. We hypothesized, therefore, that G-Gly might have a
Adenosine nucleotides acting at the human P2Y1 receptor stimulate mitogen-activated protein kinases and induce apoptosis.
Journal:J Biol Chem
PubMed ID:11278310
For the widely distributed P2Y receptors for nucleotides, the transductional and functional responses downstream of their coupling to G proteins are poorly characterized. Here we describe apoptotic induction and the associated differential stimulation of mitogen-activated protein (MAP) kinase family members by the human P2Y(1) receptor. The potent P2Y(1) receptor agonist,
Activity of adenosine diphosphates and triphosphates on a P2Y(T) -type receptor in brain capillary endothelial cells.
Journal:Br J Pharmacol
PubMed ID:11156575
1. A P2Y (nucleotide) receptor activity in a clonal population (B10) of rat brain capillary endothelial cells is coupled to inhibition of adenylyl cyclase and has functional similarities to the P2Y(T) (previously designated 'P2T') receptor for ADP of blood platelets. However, the only P2Y receptor which was detectable in a
Transcriptional induction of mitogen-activated protein kinase phosphatase 1 by retinoids. Selective roles of nuclear receptors and contribution to the antiapoptotic effect.
Journal:J Biol Chem
PubMed ID:12186877
All-trans-retinoic acid (t-RA) inhibits hydrogen peroxide (H(2)O(2))-induced apoptosis by inhibiting the c-Jun N-terminal kinase (JNK)-activator protein 1 (AP-1) pathway. In this report, we examined the involvement of mitogen-activated protein kinase phosphatase 1 (MKP-1) in suppression of JNK and the antiapoptotic effect of t-RA and the roles of nuclear receptors in
Genetic Evidence That Protease-activated Receptors Mediate Factor Xa Signaling in Endothelial Cells.
Journal:J Biol Chem
PubMed ID:11850418
The coagulation protease Factor Xa (Xa)(1) triggers a variety of cellular responses that may be important for inflammatory reactions to tissue injury. Protease-activated receptors (PAR1, PAR2, and PAR4) can mediate Xa signaling in heterologous expression systems. However, other candidate Xa receptors have been described, and the extent to which one