What additional materials do I need for my transformation?
You will need bacterial growth media, such as LB, the bacterial selection antibiotic, and induction reagent (IPTG, X-gal). We offer imMedia growth medium which is a pre-mixed, pre-sterilized medium containing selection antibiotics for growth of E. coli strains. It can be prepared with or without IPTG and X-gal.
What bacterial growth media should I use?
Check out our bacterial growth media chart (https://www.thermofisher.com/us/en/home/life-science/cell-culture/microbiological-culture/bacterial-growth-media.html).
What is the optimal pH of low salt LB for LB + blasticidin plates?
We recommend a pH of 7 or less and half the normal amount of NaCl in your LB media or plates.
See the following paper for details on optimal conditions: Yamaguchi et al (1965) Inhibition of Protein Synthesis by Blasticidin S. Journal of Biochemistry (Tokyo) Volume 57: pp 667-677.