POROS™ R1 Prepacked Reversed-Phase Columns
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Thermo Scientific™

POROS™ R1 Prepacked Reversed-Phase Columns

Thermo Scientific™ POROS™ Prepacked Reversed-Phase Column. POROS™ R1 is a 4000 angstrom pore size Poly[styrenevinylbenzene] particle, suitable for the reversed-phase separation of biomolecules.
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製品番号（カタログ番号）粒子径材料寸法数量
1101412
または、製品番号1-1014-12
10 μmStainless Steel2.1 x 30 mm1 Column
1101212
または、製品番号1-1012-12
10 μmPEEK2.1 x 30 mm1 Column
1101216
または、製品番号1-1012-16
10 μmPEEK2.1 x 100 mm1 Column
1101224
または、製品番号1-1012-24
10 μmPEEK4.6 x 50 mm1 Column
1101226
または、製品番号1-1012-26
10 μmPEEK4.6 x 100 mm1 Column
110141610 μmStainless Steel2.1 x 100 mm1 Column
1101424
または、製品番号1-1014-24
10 μmStainless Steel4.6 x 50 mm1 Column
110142610 μmStainless Steel4.6 x 100 mm1 Column
1102212
または、製品番号1-1022-12
20 μmPEEK2.1 x 30 mm1 Column
1102226
または、製品番号1-1022-26
20 μmPEEK4.6 x 100 mm1 Column
110241220 μmStainless Steel2.1 x 30 mm1 Column
1102426
または、製品番号1-1024-26
20 μmStainless Steel4.6 x 100 mm1 Column
製品番号（カタログ番号） 1101412
または、製品番号1-1014-12
価格（JPY）
171,300
Each
お問い合わせください ›
粒子径:
10 μm
材料:
Stainless Steel
寸法:
2.1 x 30 mm
数量:
1 Column

POROS R1 is a 4000 angstrom pore size Poly[styrenevinylbenzene] particle, suitable for the reversed-phase separation of biomolecules. 10 micron particle size is used for the highest resolution chromatography, and is suitable for analytical to small scale preparative separation of biomolecules. 20 micron particle size is used for high resolution and small scale preparative to semi-preparative separation of biomolecules.

POROS High Performance Chromatography™ Media and Pre-Packed Columns

  • A high performance chromatography resins for analytical to process scale separations.
  • Higher productivity: High throughput and high dynamic capacity associated with High Performance Chromatography™
  • Chemical stability: Allows aggressive cleaning and sanitization
  • Enhanced biomolecule access: Provided via large pores, ranging between 500-10,000 Å
  • Polystyrenedivinylbenzene particles: Yield a robust, easily packable matrix
  • Develop better separations methods in a shorter time frame: The speed of High Performance Chromatography technology reduces weeks of experimentation to only a few hours of work, so you have plenty of time to explore all the variables of your separation

Reduce time-consuming sample prep

You can replace many sample preparation steps with high speed High Performance Chromatography technology. For instance, dialysis of large volumes of material can be replaced by high flow rate processing of the dilute sample. Elution in a small volume of new buffer accomplishes both buffer exchange and concentration.

Create novel assays for more efficient analysis

High Performance Chromatography technology is not limited to standard modes of separation. Both enzymes and affinity ligands can be immobilized on POROS media. By combining rapid on-column protein digestions with rapid on-column immunoassays and chromatographic separations, you can create entirely new assays with unlimited potential.

High capacity, high resolution, high speed

In contrast to conventional chromatography media, POROS High Performance Chromatography™ resins particles, are engineered to have two discreet classes of pores. Large 'throughpores' allow convection flow to occur through the particles themselves, quickly carrying sample molecules to short 'diffusive' pores inside. By reducing the distance over which diffusion needs to occur, the time required for sample molecules to interact with interior binding sites is also reduced. Diffusion is no longer limiting and flow rates can be dramatically increased - without compromising resolution or capacity. Separations can be achieved at 1000 to 5000 cm/hr compared to 50 to 360 cm/hr for conventional media.

For Research Use Only. Not for use in diagnostic procedures.
仕様
寸法2.1 x 30 mm
梱包材Highly Crosslinked Styrene-Divinylbenzene polymer (ST-DVB)
数量1 Column
出荷条件Room Temperature
固定相R1
直径（メートル法）2.1 mm
長さ（メートル法）30 mm
材料Stainless Steel
粒子径10 μm
孔径4000 Å
製品ラインPOROS
タイプAnalytical Column
Unit SizeEach
組成および保存条件
Store at room temperature.

よくあるご質問（FAQ）

Are POROS analytical columns compatible with HPLC systems from Waters or Agilent?

All of the pre-packed POROS columns, regardless of column size or resin, have the same 10-32 female fittings on the column. Columns are compatible with Waters and Agilent HPLC systems. You will need 1/16 inch male fittings for the HPLC to attach the column.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

For any POROS analytical column, is the column compatible with an AKTA or Avant purification system?

All of the pre-packed columns, regardless column size or resin, have the same 10-32 female fittings on the column. They are all compatible with the AKTA instrument platform, however due to the column volume of the 2.1 mmD x 30 mmL columns (0.1 mL), these will not perform well in AKTA or Avant purification systems due to the relative size of the hold- up volume in these systems. The smallest column (PEEK or SS) that could feasibly be used in these systems is the 4.6mmD x 50mmL (0.8 mL) format.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Are the pre-packed POROS CaptureSelect or any POROS analytical columns compatible with an UPLC or is the use of these columns limited to HPLC?

UPLC is typically described as chromatography with sub-2 µm particles, which drives higher efficiency/better separation independent of operating flow rate. As particle size decreases, backpressure increases so the UPLC systems tend to have higher pressure ratings compared to HPLC systems. The flow rates on UPLC systems would be sufficient for POROS columns. Pump capabilities are typically 0.1-1.0 mL/min, so not as fast as a HPLC, but sufficient. Hold-up volumes on UPLC systems are lower than HPLCs, so this can help with sensitivity and efficiency. So from a pressure and flow standpoint there should be no challenge to operate a POROS CaptureSelect column on a UPLC system.

That being said, care should be taken running POROS columns on UPLC systems. Higher pressure safety interlock MUST be set at the rated column pressure, typically 180 bar. HPLC column hardware is not rated to operate at the static pressures possible and typical on UPLC systems. A clog or the like on a UPLC system could generate unsafe pressures on any HPLC-rated column before the default UPLC safety pressure interlock is tripped.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What is the physical or mechanical stability of POROS resins?

The physical stability of POROS resins is high. Customers have agitated, with overhead mixing and or bottom gassing for long periods of time (5-10 hours) and have performed hundreds of diaphragm pump passes during stall packs. Keep in mind that agitation with any device that creates grinding and sheer, such as with a magnetic stir bar, is not recommended for any type of resin.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What should I do if a POROS column dries out or air is pumped onto the column?

POROS resins rehydrate quite well. Here is the procedure that we recommend:

1. Flow 5% ethanol over the column at ?350 cm/hour for 3 CVs, followed by water or 0.1 M sodium chloride at 300 cm/hour for 3 CVs.
2. If baseline noise is still present, flow 20% ethanol over the column at 300 cm/hour for 3 CVs, followed by water or 0.1 M sodium chloride at 300 cm/hour for 3 CVs.
3. If you have established column qualification tests and specifications, perform the tests after rewetting.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

View all POROS™ R1 Prepacked Reversed-Phase Columns FAQs