MEM, no glutamine, 500 mL

Citations & References (1)

Gibco™

MEM, no glutamine, 500 mL

Name: MEM, no glutamine, 500 mL
SKU: 11090081

Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used詳細を見る

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製品番号（カタログ番号） 11090081

価格（JPY）

3,300

Each

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Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. We offer a variety of Gibco™ MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This MEM is modified as follows:

With	Without
• Phenol Red	• L-glutamine
	• HEPES

The complete formulation is available.

Gibco™ MEM, developed by Harry Eagle, was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow’s MEM, MEM α, DMEM, and Temin’s Modification. MEM is available with Earle’s salts for use in a CO₂ incubator, or with Hanks' salts for use without CO₂. This product is made with Earle’s salts.

MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO₂ environment to maintain physiological pH.

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.

仕様

細胞株HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts

細胞タイプPrimary Rat Astrocytes

濃度1 X

製造品質cGMP-compliant under the ISO 13485 standard

製品ラインGibco™

製品タイプMEM (Minimum Essential Medium)

数量500 mL

品質保持期間24 Months From Date of Manufacture

出荷条件Room Temperature

分類Animal Origin-free

形状Liquid

Serum LevelStandard Serum Supplementation

無菌性Sterile-filtered

添加剤ありLow Glucose, Phenol Red

添加剤なしNo Glutamine, No HEPES, No Sodium Pyruvate

Unit SizeEach

組成および保存条件

Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 24 months from date of manufacture

よくあるご質問（FAQ）

Where can I find the osmolality for MEM Medium?

The osmolality is listed in the COA for the particular lot number of the medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How can I remove mycoplasma contamination from my cell culture medium?

Very often mycoplasma contamination cannot be removed from the culture so it should be discarded. You may have a unique culture that you prefer not to discard and would like to try to clean it. Ciprofloxacin and Plasmocin have reportedly been used for this application. If interested in a protocol or directions for use, check with the antibiotic supplier or published literature. Note that mycoplasma are very difficult to remove from culture and spread easily so the treated cultures should be quarantined until clear of mycoplasma, and your laboratory should be thoroughly cleaned.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I see a decrease in growth of my culture. What should I do?

Try changing the medium or serum. Compare media formulations for differences in glucose, amino acids, and other components. Compare an old lot of serum with a new lot. Increase initial cell inoculums. Lastly, adapt cells sequentially to new medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all MEM, no glutamine, 500 mL FAQs

引用および参考文献 (1)

引用および参考文献

Abstract

Myocardin expression is regulated by nkx2.5, and its function is required for cardiomyogenesis.

Authors:Ueyama T, Kasahara H, Ishiwata T, Nie Q, Izumo S,

Journal:Mol Cell Biol

PubMed ID:14645532

Nkx2.5 (also known as Csx) is an evolutionarily conserved cardiac transcription factor of the homeobox gene family. Nkx2.5 is required for early heart development, since Nkx2.5-null mice die before completion of cardiac looping. To identify genes regulated by Nkx2.5 in the developing heart, we performed subtractive hybridization by using RNA ... More