Platinum™ Taq DNA Polymerase, High Fidelity
Platinum&trade; <i>Taq</i> DNA Polymerase, High Fidelity
Citations & References (4)
Invitrogen™

Platinum™ Taq DNA Polymerase, High Fidelity

Platinum™ Taq DNAポリメラーゼ高忠実度は、高収率と堅牢な増幅が必要な場合のDNAフラグメントの増幅に最適です。高い忠実度は、Platinum™ Taq DNAポリメラーゼとプルーフリーディング（3´→5´エキソヌクレアーゼ活性）酵素詳細を見る
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製品番号（カタログ番号）反応数
11304029500 Reactions
11304011反応100回分
113041025000 Reactions
製品番号（カタログ番号） 11304029
価格（JPY）
59,500
Online offer
Ends: 26-Dec-2025
99,300
割引額 39,800 (40%)
Each
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反応数:
500 Reactions
一括またはカスタム形式をリクエストする
Platinum™ Taq DNAポリメラーゼ高忠実度は、高収率と堅牢な増幅が必要な場合のDNAフラグメントの増幅に最適です。高い忠実度は、Platinum™ Taq DNAポリメラーゼとプルーフリーディング（3´→5´エキソヌクレアーゼ活性）酵素、Pyrococcus種のGB-Dポリメラーゼの混合物によるものです。Platinum™自動ホットスタート技術を採用することで、PCR特異性が向上します。Platinum™ Taq DNAポリメラーゼ、高忠実度の特長：

•忠実度—Taq DNAポリメラーゼの6倍以上の忠実度
• アンプリコンサイズ—最大15 kbのフラグメントの増幅（図参照）
• 利便性—室温での反応組み立て
• アプリケーション—複雑なゲノム、ウイルス、プラスミドテンプレートから得られたDNA増幅、およびRT-PCR

ユニット定義
忠実度の高いPlatinum™ Taq DNAポリメラーゼの1ユニットは、74°℃、30分の条件下で、デオキシリボヌクレアーゼの10 nmolesをDNAに組み込むために必要な酵素の量です。
研究用にのみ使用できます。診断用には使用いただけません。
仕様
フィデリティ（vs. Taq）6X
ホットスタート内蔵ホットスタート
反応数500 Reactions
オーバーハング混合型
ポリメラーゼPlatinum Taq DNAポリメラーゼハイフィデリティ
製品タイプDNAポリメラーゼハイフィデリティ
数量500 rxns
反応形態別のコンポーネント
出荷条件ドライアイス
サイズ（最終製品）20 kb以下
検出法プライマー-プローブ
使用対象（アプリケーション）Hot-start PCR, High-fidelity PCR
GC-Rich PCR Performance低
反応速度スタンダード
Unit SizeEach
組成および保存条件
• Platinum Taq DNAポリメラーゼハイフィデリティ（5 U/µL）、1 x 100 µL
• 10Xハイフィデリティバッファー[600 mM Tris-SO4（pH 8.9）、180 mM（NH4）2SO4]、1 x 2.25 mL
• 50 mM MgSO4、1 x 1 mL

-10℃～-30℃で保存。

よくあるご質問（FAQ）

My oligonucleotide does not appear to be the right length when I checked by gel electrophoresis. Why is this?

Oligos should be run on a polyacrylamide gel containing 7 M urea and loaded with a 50% formamide solution to avoid compressions and secondary structures. Oligos of the same length and different compositions can electrophorese differently. dC's migrate fastest, followed by dA's, dT's, and then dG's. Oligos containing N's tend to run as a blurry band and generally have a problem with secondary structure.

The primers I am using worked for PCR initially, but over time, have stopped working. What happened?

Primers should be aliquoted for single use before PCR set-up. Heat just the aliquoted primers to 94 degrees for 1 min. Quick chill the primer on ice before adding to the PCR reaction. Some primers may anneal to themselves or curl up on themselves.

I don't see a pellet in my oligo tube order. Should I ask for a replacement?

The drying method dries the primer in a thin layer along the sidewalls of the tube instead of the bottom, therefore a pellet is not always visible and should still be ready to use.

There is a ball-shaped pellet at the bottom of my oligo tube. What is this and can I still use my oligo?

If the oligo was overheated, it will appear as a “ball”-shaped pellet attached to the bottom of the tube. This should not affect the quality of the oligo, and the oligo should be readily soluble in water.

There is a green color in my lyophilized oligo. Can I still use it?

If an oligo appears green in color, this is most likely due to ink falling into the tube. The oligo should still be fully functional. The color can be removed by doing an ethanol precipitation.

View all Platinum™ Taq DNA Polymerase, High Fidelity FAQs

引用および参考文献 (4)

引用および参考文献
Abstract
Relation between kidney function, proteinuria, and adverse outcomes.
Authors:Hemmelgarn BR, Manns BJ, Lloyd A, James MT, Klarenbach S, Quinn RR, Wiebe N, Tonelli M
Journal:JAMA
PubMed ID:20124537
'The current staging system for chronic kidney disease is based primarily on estimated glomerular filtration rate (eGFR) with lower eGFR associated with higher risk of adverse outcomes. Although proteinuria is also associated with adverse outcomes, it is not used to refine risk estimates of adverse events in this current system.' ... More
Factor B and the mitochondrial ATP synthase complex.
Authors: Belogrudov Grigory I; Hatefi Youssef;
Journal:J Biol Chem
PubMed ID:11744738
Factor B is a subunit of the mammalian ATP synthase complex, whose existence has been controversial. This paper describes the molecular and functional properties of a recombinant human factor B, which when added to bovine submitochondrial particles depleted of their factor B restores the energy coupling activity of the ATP ... More
Human protein factory for converting the transcriptome into an in vitro-expressed proteome,.
Authors:Goshima N, Kawamura Y, Fukumoto A, Miura A, Honma R, Satoh R, Wakamatsu A, Yamamoto J, Kimura K, Nishikawa T, Andoh T, Iida Y, Ishikawa K, Ito E, Kagawa N, Kaminaga C, Kanehori K, Kawakami B, Kenmochi K, Kimura R, Kobayashi M, Kuroita T, Kuwayama H, Maruyama Y, Matsuo K, Minami K, Mitsubori M, Mori M, Morishita R, Murase A, Nishikawa A, Nishikawa S, Okamoto T, Sakagami N, Sakamoto Y, Sasaki Y, Seki T, Sono S, Sugiyama A, Sumiya T, Takayama T, Takayama Y, Takeda H, Togashi T, Yahata K, Yamada H,
Journal:Nat Methods
PubMed ID:19054851
Appropriate resources and expression technology necessary for human proteomics on a whole-proteome scale are being developed. We prepared a foundation for simple and efficient production of human proteins using the versatile Gateway vector system. We generated 33,275 human Gateway entry clones for protein synthesis, developed mRNA expression protocols for them ... More
Mutation screening in 86 known X-linked mental retardation genes by droplet-based multiplex PCR and massive parallel sequencing.
Authors:Hu H, Wrogemann K, Kalscheuer V, Tzschach A, Richard H, Haas SA, Menzel C, Bienek M, Froyen G, Raynaud M, Van Bokhoven H, Chelly J, Ropers H, Chen W
Journal:Hugo J
PubMed ID:21836662
Massive parallel sequencing has revolutionized the search for pathogenic variants in the human genome, but for routine diagnosis, re-sequencing of the complete human genome in a large cohort of patients is still far too expensive. Recently, novel genome partitioning methods have been developed that allow to target re-sequencing to specific ... More