Ham's F-10 Nutrient Mix
Ham's F-10 Nutrient Mix
Citations & References (2)
Gibco™

Ham's F-10 Nutrient Mix

Ham's F-10 Nutrient Mixture (F-10) was designed for serum-free growth of Chinese Hamster Ovary (CHO) cells. F-10 has since been詳細を見る
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製品番号（カタログ番号）数量
11550043500 mL
製品番号（カタログ番号） 11550043
価格（JPY）
3,300
Each
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数量:
500 mL
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Ham's F-10 Nutrient Mixture (F-10) was designed for serum-free growth of Chinese Hamster Ovary (CHO) cells. F-10 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including COS-7, primary rat astrocytes, and rat prostate epithelial cells.

This Ham's F-10 is modified as follows:
WithWithout
• Phenol Red• HEPES
• L-glutamine 


The complete formulation is available.

Using Ham's F-10
Compared to other basal media, F-10 contains a wider variety of components, including zinc, hypoxanthine, and thymidine. Serum-free growth of CHO cells in F-10 has led to a variety of improved formulations, such as Ham's F-12 Nutrient Mixture (F-12). F-10 contains no proteins or growth factors. Therefore, F-10 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). F-10 uses a sodium bicarbonate buffer system (1.2 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

研究用途にのみご使用ください。診断目的には使用できません。
仕様
細胞株CHO, COS-7, and rat prostate epithelial cells
細胞タイプPrimary Rat Astrocytes
濃度1 X
製造品質cGMP-compliant under the ISO 13485 standard
製品ラインGibco
製品タイプHam's F-10 Nutrient Mixture
数量500 mL
品質保持期間12 Months From Date of Manufacture
出荷条件Room Temperature
分類Animal Origin-free
形状Liquid
無菌性Sterile-filtered
添加剤ありHigh Glucose, Glutamine, Phenol Red, Sodium Pyruvate
添加剤なしNo HEPES
Unit SizeEach
組成および保存条件
Storage conditions: 2°C to 8°C (protect from light)
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

よくあるご質問（FAQ）

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How can I remove mycoplasma contamination from my cell culture medium?

Very often mycoplasma contamination cannot be removed from the culture so it should be discarded. You may have a unique culture that you prefer not to discard and would like to try to clean it. Ciprofloxacin and Plasmocin have reportedly been used for this application. If interested in a protocol or directions for use, check with the antibiotic supplier or published literature. Note that mycoplasma are very difficult to remove from culture and spread easily so the treated cultures should be quarantined until clear of mycoplasma, and your laboratory should be thoroughly cleaned.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I see a decrease in growth of my culture. What should I do?

Try changing the medium or serum. Compare media formulations for differences in glucose, amino acids, and other components. Compare an old lot of serum with a new lot. Increase initial cell inoculums. Lastly, adapt cells sequentially to new medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

My cells are not adhering to the culture vessel. What should I do?

This can occur if cells are overly trypsinized. Trypsinize for a shorter time or use less trypsin. Mycoplasma contamination could also cause this problem. Segregate your culture and test for mycoplasma infection. Lastly, check for attachment factors in the medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Ham's F-10 Nutrient Mix FAQs

引用および参考文献 (2)

引用および参考文献
Abstract
Subretinal transplantation of genetically modified human cell lines attenuates loss of visual function in dystrophic rats.
Authors: Lund R D; Adamson P; Sauvé Y; Keegan D J; Girman S V; Wang S; Winton H; Kanuga N; Kwan A S; Beauchène L; Zerbib A; Hetherington L; Couraud P O; Coffey P; Greenwood J;
Journal:Proc Natl Acad Sci U S A
PubMed ID:11504951
'Royal College of Surgeons rats are genetically predisposed to undergo significant visual loss caused by a primary dysfunction of retinal pigment epithelial (RPE) cells. By using this model, we have examined the efficacy of subretinal transplantation of two independent human RPE cell lines each exhibiting genetic modifications that confer long-term ... More
Sp1- and Sp3-mediated transcriptional regulation of the fibroblast growth factor receptor 1 gene in chicken skeletal muscle cells.
Authors: Parakati Rajini; DiMario Joseph X;
Journal:J Biol Chem
PubMed ID:11756440
Expression of the fibroblast growth factor receptor 1 (FGFR1) gene in skeletal muscle is positively regulated in proliferating myoblasts and declines during differentiation. We have characterized the cis-regulatory elements in the proximal region of the FGFR1 promoter which render positive transcriptional activity. Multiple elements between -69 and -14 activate the ... More